LTBR Protein (AA 31-227) (Fc Tag)

Catalog No. ABIN7275218

Product Details

Target: LTBR (Lymphotoxin beta Receptor (TNFR Superfamily, Member 3) (LTBR))

Protein Type: Recombinant

Protein Characteristics: AA 31-227

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This LTBR protein is labelled with Fc Tag.

Purpose: Human LTBR Protein

Sequence: Gln31-Met227

Characteristics: Recombinant Human LTBR Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Gln31-Met227.

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human LIGHT, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Human LTBR, hFc Tag with the EC50 22.9ng/ml determined by ELISA. See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: LTBR (Lymphotoxin beta Receptor (TNFR Superfamily, Member 3) (LTBR))

Alternative Name: LTBR (LTBR Products)

Synonyms: AI256028 Protein, CD18 Protein, LTbetaR Protein, Ltar Protein, TNF-R-III Protein, TNFCR Protein, TNFR-RP Protein, TNFR2-RP Protein, TNFRrp Protein, Tnfbr Protein, Tnfrsf3 Protein, D12S370 Protein, LT-BETA-R Protein, TNFR3 Protein, TNFRSF3 Protein, lymphotoxin beta receptor Protein, lymphotoxin B receptor Protein, Ltbr Protein, LTBR Protein

Background: Components of lymphotoxin beta receptor (LTBR)-associated signaling complexes, including TRAF2, TRAF3, NIK, IKK1, and IKK2 have been shown to participate in the coupling of LTBR to NFkappaB.TRAF3 functions as a negative regulator of LTBR signaling via both canonical and non-canonical NFkappaB pathways by two distinct mechanisms. These effects of TRAF3 depletion did not require LTBR signaling and were consistent with autonomous activation of the non-canonical NFkappaB pathway.

Molecular Weight: 48.5 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result.

Pathways: NF-kappaB Signaling