MADCAM1 Protein (AA 19-317) (His tag)
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- Target See all MADCAM1 Proteins
- MADCAM1 (Mucosal Vascular Addressin Cell Adhesion Molecule 1 (MADCAM1))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 19-317
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This MADCAM1 protein is labelled with His tag.
- Purpose
- Human MADCAM1 Protein
- Sequence
- Gln19-Gln317
- Characteristics
- Recombinant Human MADCAM1 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln19-Gln317.
- Purity
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human MADCAM1, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-MADCAM1 Antibody, hFc Tag with the EC50 of 7.7ng/ml determined by ELISA. See testing image for detail.
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Mucosal Vascular Addressin Cell Adhesion Molecule 1 (MADCAM1) (AA 22-364) protein (His tag)
MADCAM1 Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Mucosal Vascular Addressin Cell Adhesion Molecule 1 (MADCAM1) (Transcript Variant 2) protein (Myc-DYKDDDDK Tag)MADCAM1 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- MADCAM1 (Mucosal Vascular Addressin Cell Adhesion Molecule 1 (MADCAM1))
- Alternative Name
- MADCAM1 (MADCAM1 Products)
- Synonyms
- MACAM1 Protein, AV211525 Protein, mucosal vascular addressin cell adhesion molecule 1 Protein, MADCAM1 Protein, Madcam1 Protein
- Background
- Mucosal addressin cell adhesion molecule-1 (MAdCAM-1) contributes to the recruitment of donor T cells into the mucosal tissues of the recipient after allogeneic hematopoietic stem cell transplantation (aHSCT). The aim of our study was to determine whether selected single nucleotide polymorphisms (SNPs) of the MADCAM1 gene are associated with development of serious complications after aHSCT.
- Molecular Weight
- 32.5 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result.
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