MMP 9 Protein (AA 20-706) (His tag)

Catalog No. ABIN7275266

Product Details

Target: MMP 9 (MMP9) (Matrix Metallopeptidase 9 (Gelatinase B, 92kDa Gelatinase, 92kDa Type IV Collagenase) (MMP9))

Protein Type: Recombinant

Protein Characteristics: AA 20-706

Origin: Cynomolgus

Source: HEK-293 Cells

Purification tag / Conjugate: This MMP 9 protein is labelled with His tag.

Purpose: Cynomolgus MMP-9 Protein

Sequence: Ala20-Asp706

Characteristics: Recombinant Cynomolgus MMP-9 Protein is expressed from HEK293 with His tag at the C-Terminus. The protein needs to be activated by APMA to have hydrolytic activity.It contains Ala20-Asp706.

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Cynomolgus MMP-9, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-MMP-9 Antibody, hFc Tag with the EC50 of 18.8ng/ml determined by ELISA. See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: MMP 9 (MMP9) (Matrix Metallopeptidase 9 (Gelatinase B, 92kDa Gelatinase, 92kDa Type IV Collagenase) (MMP9))

Alternative Name: MMP-9 (MMP9 Products)

Synonyms: CLG4B Protein, GELB Protein, MANDP2 Protein, MMP-9 Protein, AW743869 Protein, B/MMP9 Protein, Clg4b Protein, pro-MMP-9 Protein, mmp-9 Protein, fgMMP-9 Protein, mmp9 Protein, ZFMMP-9 Protein, wu:fb02g06 Protein, wu:fb07b05 Protein, wu:fi98c09 Protein, wu:fj05a08 Protein, zgc:64165 Protein, clg4b Protein, gelb Protein, mandp2 Protein, matrix metallopeptidase 9 Protein, collagenase Protein, matrix metalloproteinase 9 Protein, matrix metallopeptidase 9 S homeolog Protein, MMP9 Protein, RB3913 Protein, BPSS0666 Protein, Sbal_3732 Protein, Bcer98_0486 Protein, Shew185_0630 Protein, Shal_3056 Protein, Sbal195_0657 Protein, Lbys_3550 Protein, Palpr_2084 Protein, Mmp9 Protein, mmp9 Protein, mmp9.S Protein

Background: Matrix metalloproteinase 9 (MMP9) contributes to this process and deficiencies in the MMP9 lead to impaired healing. Inappropriate expression of MMP9 also contributes to impaired re-epithelialization. Previously we demonstrated that FOXO1 was activated in wound healing but to higher levels in diabetic wounds. To address mechanisms of impaired re-epithelialization we examined MMP9 expression in vivo in full thickness dermal scalp wounds created in experimental K14.

Molecular Weight: 77.44 kDa. Due to glycosylation, the protein migrates to 85-100 kDa based on Tris-Bis PAGE result.

UniProt: A0A2K5UU71

Pathways: Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, CXCR4-mediated Signaling Events