MUC16 Protein (AA 12660-12923) (His-Avi Tag)

Catalog No. ABIN7275299

Product Details

Target: MUC16 (CA125) (Mucin 16, Cell Surface Associated (CA125))

Protein Type: Recombinant

Protein Characteristics: AA 12660-12923

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This MUC16 protein is labelled with His-Avi Tag.

Purpose: Human CA125/MUC16 Protein

Sequence: Gly12660-Met12923

Characteristics: Recombinant Human CA125/MUC16 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly12660-Met12923.

Purity: > 95 % as determined by Tris-Bis PAGE

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human CA125, His Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Human MSLN, hFc Tag with the EC50 of 2.8μg/ml determined by ELISA. See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: MUC16 (CA125) (Mucin 16, Cell Surface Associated (CA125))

Alternative Name: CA125 (CA125 Products)

Synonyms: CA125 Protein, 1110008I14Rik Protein, Ca125 Protein, Gm1480 Protein, RGD1308422 Protein, MUC16 Protein, mucin 16, cell surface associated Protein, mucin 16 Protein, mucin-16 Protein, MUC16 Protein, Muc16 Protein, LOC476718 Protein, LOC712735 Protein

Background: MUC16, also known as the CA125 antigen, is a mucin protein that may be found in type I transmembrane or secreted forms that are used monitor the progress of epithelial ovarian cancer therapy.Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces.Binding to MSLN mediates heterotypic cell adhesion. This may contribute to the metastasis of ovarian cancer to the peritoneum by initiating cell attachment to the mesothelial epithelium via binding to MSLN.

Molecular Weight: 31.3 kDa. Due to glycosylation, the protein migrates to 50-80 kDa based on Tris-Bis PAGE result.

UniProt: Q8WXI7