NOTCH2 Protein (AA 26-530) (Biotin,His-Avi Tag)

Catalog No. ABIN7275365

Recombinant NOTCH2 protein expressed in HEK-293 Cells.

Quick Overview for NOTCH2 Protein (AA 26-530) (Biotin,His-Avi Tag) (ABIN7275365)

Target: NOTCH2 (Notch 2 (NOTCH2))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 26-530

Purification tag / Conjugate: This NOTCH2 protein is labelled with Biotin,His-Avi Tag.

Purpose: Biotinylated Human Notch 2 Protein

Sequence: Leu26-Gln530

Characteristics: Recombinant Biotinylated Human Notch 2 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Leu26-Gln530.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Biotinylated Human Notch 2 on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.,The purity of Biotinylated Human Notch 2 is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: NOTCH2 (Notch 2 (NOTCH2))

Alternative Name: Notch 2

Background: Human Notch-2 is a 300 kDa type I transmembrane glycoprotein that is one of four human Notch homologues involved in developmental processes. Although Notch proteins are structurally and functionally similar, deletion of either Notch-1 or Notch-2 is lethal, showing that not all functions overlap.Notch-2 functions as a receptor for membrane-bound ligands Jagged-1 (JAG1), Jagged-2 (JAG2) and Delta-1 (DLL1) to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus.

Molecular Weight: 57 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Bis-Tris PAGE result.

UniProt: Q04721

Pathways: Notch Signaling, Stem Cell Maintenance