Neurotensin Protein (NTS) (AA 24-163) (Fc Tag)
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- Target See all Neurotensin (NTS) Proteins
- Neurotensin (NTS)
- Protein Type
- Recombinant
- Protein Characteristics
- AA 24-163
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This Neurotensin protein is labelled with Fc Tag.
- Purpose
- Human NTS1 Protein
- Sequence
- Ser24-Leu163
- Characteristics
- Recombinant Human NTS1 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Ser24-Leu163.
- Purity
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human NTS1, hFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Anti-NTS Antibody, hFc Tag with the EC50 of 0.17μg/ml determined by ELISA. See testing image for detail.
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Neurotensin (NTS) (AA 23-162) protein (Fc Tag)
NTS Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
Neurotensin (NTS) (AA 71-170) protein (GST tag)NTS Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Neurotensin (NTS) (AA 1-170) protein (GST tag)NTS Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Neurotensin (NTS) (AA 23-147) protein (His tag)Crystallography grade NTS Origin: Mouse Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- Neurotensin (NTS)
- Alternative Name
- NTS1 (NTS Products)
- Synonyms
- NTS Protein, NMN-125 Protein, NN Protein, NT Protein, NT/N Protein, NTS1 Protein, 5033428E16Rik Protein, neurotensin Protein, NTS Protein, Nts Protein
- Background
- Crystal structures of neurotensin receptor subtype 1 (NTS1) allowed us to visualize the binding mode of the endogenous peptide hormone neurotensin and its pharmacologically active C-terminal fragment NT(8-13) within the orthosteric binding pocket of NTS1.
- Molecular Weight
- 42.9 kDa. Due to glycosylation, the protein migrates to 48-53 kDa based on Tris-Bis PAGE result.
- UniProt
- P30990
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