PDGFRB Protein (AA 33-530) (His-Avi Tag)

Catalog No. ABIN7275445

Product Details

Target: PDGFRB (Platelet Derived Growth Factor Receptor beta (PDGFRB))

Protein Type: Recombinant

Protein Characteristics: AA 33-530

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This PDGFRB protein is labelled with His-Avi Tag.

Purpose: Human PDGF R beta/CD140b Protein

Sequence: Leu33-Phe530

Characteristics: Recombinant Human PDGF R beta/CD140b Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Leu33-Phe530.

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: PDGFRB (Platelet Derived Growth Factor Receptor beta (PDGFRB))

Alternative Name: PDGF R beta (PDGFRB Products)

Synonyms: AI528809 Protein, CD140b Protein, PDGFR-1 Protein, Pdgfr Protein, CD140B Protein, IBGC4 Protein, JTK12 Protein, PDGFR Protein, PDGFR1 Protein, platelet derived growth factor receptor, beta polypeptide Protein, platelet derived growth factor receptor beta Protein, Pdgfrb Protein, PDGFRB Protein

Background: PDGF is a major serum mitogen that can exist as a homo or hetero-dimeric protein consisting of disulfide-linked PDGF-A and PDGF-B chains. The PDGF-AA,PDGF-BB and PDGF-AB isoforms have been shown to bind to two distinct cell surface PDGF receptors with different affinities. Where as PDGF R alpha binds all three PDGF isoforms with high affinity, PDGF R beta binds PDGF-BB only with high-affinity. PDGF R plays an essential role in blood vessel development by promoting proliferation, migration and recruitment of pericytes and smooth muscle cells to endothelial cells. Plays a role in the migration of vascular smooth muscle cells and the formation of neointima at vascular injury sites.

Molecular Weight: 59 kDa. Due to glycosylation, the protein migrates to 78-115 kDa based on Tris-Bis PAGE result.

UniProt: P09619

Pathways: Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Inositol Metabolic Process, Glycosaminoglycan Metabolic Process, Smooth Muscle Cell Migration, Platelet-derived growth Factor Receptor Signaling