Phospholipase D4 Protein (PLD4) (AA 58-503) (His tag)
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- Target See all Phospholipase D4 (PLD4) Proteins
- Phospholipase D4 (PLD4)
- Protein Type
- Recombinant
- Protein Characteristics
- AA 58-503
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Origin
- Mouse
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This Phospholipase D4 protein is labelled with His tag.
- Purpose
- Mouse PLD4 Protein
- Sequence
- Gln58-Trp503
- Characteristics
- Recombinant Mouse PLD4 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln58-Trp503.
- Purity
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
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Phospholipase D4 (PLD4) (AA 52-506) protein (His tag)
PLD4 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Phospholipase D4 (PLD4) (AA 1-489) protein (GST tag)PLD4 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Phospholipase D4 (PLD4) (AA 1-506) protein (Strep Tag)Crystallography grade PLD4 Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, ELISA, WB
Phospholipase D4 (PLD4) (AA 1-503) protein (Strep Tag)Crystallography grade PLD4 Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, ELISA, WB
Phospholipase D4 (PLD4) (AA 52-506), (partial) protein (His-SUMO Tag)PLD4 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % SDS
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in 50 mM MES, 100 mM NaCl ( pH 6.0).
- Buffer
- Lyophilized from 0.22μm filtered solution in 50 mM MES, 100 mM NaCl ( pH 6.0). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- Phospholipase D4 (PLD4)
- Alternative Name
- PLD4 (PLD4 Products)
- Synonyms
- C14orf175 Protein, AI132321 Protein, thss Protein, phospholipase D family member 4 Protein, phospholipase D family, member 4 Protein, PLD4 Protein, Pld4 Protein
- Background
- Phospholipase D4 (PLD4) is a newly identified protein expressed in microglia. the expression of PLD4 was located in macrophages in the colon cancer mesenchymal and lymph nodes as shown by immunohistochemical analysis. furthermore, its expression was associated with clinical staging of colon cancer. Then, THP-1 as a cell model induced into TAMs. PLD4 could be involved in the activation process of M1 phenotype macrophages.
- Molecular Weight
- 51 kDa. Due to glycosylation, the protein migrates to 60-80 kDa based on Tris-Bis PAGE result.
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