SARS-CoV-2 Spike S1 Protein (Fc-Avi Tag)

Catalog No. ABIN7275552

This Recombinant SARS-CoV-2 Spike S1 protein is produced in HEK-293 Cells.

Quick Overview for SARS-CoV-2 Spike S1 Protein (Fc-Avi Tag) (ABIN7275552)

Target: SARS-CoV-2 Spike S1

Protein Type: Recombinant

Origin: SARS Coronavirus (SARS-CoV)

Source: HEK-293 Cells

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Product Details

Purification tag / Conjugate: This SARS-CoV-2 Spike S1 protein is labelled with Fc-Avi Tag.

Purpose: SARS Spike S1 Protein

Sequence: Ser14-Arg667

Characteristics: Recombinant SARS spike S1 protein is expressed from HEK293 with hFc tag and Avi tag at the C-Terminus.It contains Ser14-Arg667.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human ACE2 at 2μg/ml (100μl/Well).Dose response curve for SARS Spike S1, hFc Tag with the EC50 of 0.39μg/ml determined by ELISA. See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: SARS-CoV-2 Spike S1

Alternative Name: SARS-CoV Spike S1

Background: The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates this interaction.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

Molecular Weight: 100.6 kDa. Due to glycosylation, the protein migrates to 120-140 kDa based on Tris-Bis PAGE result.

UniProt: P59594