SIRP AlphaV2 (AA 31-369) protein (His-Avi Tag,Biotin)
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- Target
- SIRP AlphaV2
- Protein Type
- Recombinant
- Protein Characteristics
- AA 31-369
- Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- His-Avi Tag,Biotin
- Purpose
- Biotinylated Human SIRP alpha V2/CD172a Protein
- Sequence
- Glu31-Arg369
- Characteristics
- Recombinant Biotinylated Human SIRP alpha V2/CD172a Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Glu31-Arg369.
- Purity
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Biotinylated Human SIRP alpha V2, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Human CD47, hFc Tag with the EC50 of 0.11μng/ml determined by ELISA. See testing image for detail.
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SIRP AlphaV2 protein (His tag)
Origin: Human Host: HEK-293 Cells Recombinant > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
SIRP AlphaV2 protein (Fc Tag)Origin: Human Host: HEK-293 Cells Recombinant > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
SIRP AlphaV2 (AA 31-369) (Active) protein (His tag)Origin: Human Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
SIRP AlphaV2 (AA 31-369) (Active) protein (His tag,AVI tag,Biotin)Origin: Human Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- SIRP AlphaV2
- Alternative Name
- SIRP alpha V2
- Background
- Signal regulatory protein α (SIRPα) is a regulatory membrane glycoprotein from SIRP family expressed mainly by myeloid cells and also by stem cells or neurons.SIRPα acts as inhibitory receptor and interacts with a broadly expressed transmembrane protein CD47 also called the "don´t eat me" signal.Cancer cells highly expressed CD47 that activate SIRP α and inhibit macrophage-mediated destruction.
- Molecular Weight
- 40 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Tris-Bis PAGE result.
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