TIGIT Protein (His-Avi Tag,Biotin)
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- Target See all TIGIT Proteins
- TIGIT (T Cell Immunoreceptor with Ig and ITIM Domains (TIGIT))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This TIGIT protein is labelled with His-Avi Tag,Biotin.
- Sequence
- Met22-Pro141
- Purity
- > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Biotinylated Human TIGIT, His Tag at 0.5μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Human CD155, hFc Tag with the EC50 of 8.7ng/ml determined by ELISA. See testing image for detail.
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- Discover our top product TIGIT Protein
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T Cell Immunoreceptor with Ig and ITIM Domains (TIGIT) (AA 22-141) protein (Fc Tag)
TIGIT Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by SEC-HPLC
T Cell Immunoreceptor with Ig and ITIM Domains (TIGIT) (AA 22-141) protein (His tag)TIGIT Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
T Cell Immunoreceptor with Ig and ITIM Domains (TIGIT) (AA 26-143) protein (Fc Tag)TIGIT Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
T Cell Immunoreceptor with Ig and ITIM Domains (TIGIT) (AA 26-143) protein (His tag)TIGIT Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
T Cell Immunoreceptor with Ig and ITIM Domains (TIGIT) (AA 22-141) protein (His tag)TIGIT Origin: Cynomolgus, Rhesus Monkey Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
T Cell Immunoreceptor with Ig and ITIM Domains (TIGIT) protein (mFc Tag)TIGIT Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. ELISA
T Cell Immunoreceptor with Ig and ITIM Domains (TIGIT) (AA 22-141) (Active) protein (Fc Tag)TIGIT Origin: Human Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
T Cell Immunoreceptor with Ig and ITIM Domains (TIGIT) (AA 89-208) (Active) protein (His tag)TIGIT Origin: Cynomolgus, Rhesus Monkey Host: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Func Active
T Cell Immunoreceptor with Ig and ITIM Domains (TIGIT) (AA 22-141) (Active) protein (Fc Tag,AVI tag,Biotin)TIGIT Origin: Human Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Func Active
T Cell Immunoreceptor with Ig and ITIM Domains (TIGIT) protein (Fc Tag)TIGIT Origin: Cynomolgus Host: Human Cells Recombinant > 95 % as determined by reducing SDS-PAGE.
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.
- Storage
- 4 °C,-80 °C
- Storage Comment
- Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- TIGIT (T Cell Immunoreceptor with Ig and ITIM Domains (TIGIT))
- Alternative Name
- TIGIT (TIGIT Products)
- Synonyms
- VSIG9 Protein, VSTM3 Protein, WUCAM Protein, ENSMUSG00000071552 Protein, Vstm3 Protein, RGD1563191 Protein, T-cell immunoreceptor with Ig and ITIM domains Protein, T cell immunoreceptor with Ig and ITIM domains Protein, TIGIT Protein, Tigit Protein
- Background
- TIGIT, VSIG9, VSTM3, WUCAM,TIGIT, also called Vstm3, Vsig9, and WUCAM, is a transmembrane protein in the CD28 family of the Ig superfamily proteins.TIGIT is expressed at low levels on peripheral memory and regulatory CD4 T-cells and NK cells and is up-regulated following activation of these cells.
- Molecular Weight
- 16.1 kDa. Due to glycosylation, the protein migrates to 20-25 kDa based on Tris-Bis PAGE result.
- UniProt
- Q495A1
- Pathways
- Cancer Immune Checkpoints
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