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CD47 Protein (CD47)

Synthetic Nanodisc CD47 protein expressed in HEK-293 Cells.
Catalog No. ABIN7491584

Quick Overview for CD47 Protein (CD47) (ABIN7491584)

Target

See all CD47 Proteins
CD47

Protein Type

Synthetic Nanodisc

Origin

  • 35
  • 15
  • 6
  • 6
  • 4
  • 2
  • 1
Human

Source

  • 41
  • 8
  • 6
  • 3
  • 3
  • 2
  • 1
  • 1
HEK-293 Cells
  • Purpose

    Human CD47 full length protein-synthetic nanodisc

    Characteristics

    Unlike other membrane scaffold protein (MSP) Nanodisc on the market, our synthetic Nanodisc can be prepared directly from the cells. The polymers used during this process have a dual function. It dissolves the cell membranes, like the detergent, and uses cellular phospholipids to form Nanodisc around the membrane proteins. The target protein embedded Nanodiscs can then be purified.
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  • Comment

    Advantages of Synthetic Nanodiscs:

    • Highly purified membrane proteins
    • High solubility in aqueous solutions
    • High stability
    • Proteins are in a native membrane environment and remain biologically active
    • No detergent and can be used for cell-based assays
    • No MSP backbone proteins
    Limitations of Synthetic Nanodiscs:
    • Intolerant to acids and high concentrations of divalent metal ions

    Restrictions

    For Research Use only
  • Format

    Lyophilized

    Buffer

    Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0).  Normally 5 % - 8 % trehalose is added as protectants before lyophilization.

    Storage

    -20 °C,-80 °C

    Storage Comment

    Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

    Expiry Date

    12 months
  • Target

    CD47

    Alternative Name

    CD47

    Background

    A membrane protein involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix. The encoded protein is also a receptor for the C-terminal cell binding domain of thrombospondin, and it may play a role in membrane transport and signal transduction. This gene has broad tissue distribution, and is reduced in expression on Rh erythrocytes.

    Molecular Weight

    The human full length CD47 protein has a MW of 35.2 kDa

    UniProt

    Q08722
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