G Protein-Coupled Receptor 133 Protein (GPR133)
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- Target See all G Protein-Coupled Receptor 133 (GPR133) Proteins
- G Protein-Coupled Receptor 133 (GPR133)
- Protein Type
- Synthetic
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Origin
- Human
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Source
- HEK-293 Cells
- Purpose
- Human ADGRD1 full length protein-synthetic nanodisc
- Characteristics
- Full Length Transmembrane Proteins (synthetic Nanodisc)
- Top Product
- Discover our top product GPR133 Protein
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G Protein-Coupled Receptor 133 (GPR133) (AA 26-570) protein (His tag)
Crystallography grade GPR133 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. Crys, ELISA, SDS, WB
G Protein-Coupled Receptor 133 (GPR133) (AA 27-599) protein (His tag)Crystallography grade GPR133 Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. Crys, ELISA, SDS, WB
G Protein-Coupled Receptor 133 (GPR133) (AA 26-874) protein (rho-1D4 tag)Crystallography grade GPR133 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. Crys, ELISA, SDS, WB
G Protein-Coupled Receptor 133 (GPR133) (AA 27-903) protein (rho-1D4 tag)Crystallography grade GPR133 Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. Crys, ELISA, SDS, WB
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- Application Notes
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- Applications for VLPs:
- ELISA
- SPR affinity analysis
- Phage display screening
- Immunization
- Cell based assays
- CAR-T cell screening
- Protein cystal structure analysis
- Comment
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Synthetic Nanodisc can be prepared directly from the cells. The polymers used during this process have a dual function. It dissolves the cell membranes, like the detergent, and uses cellular phospholipids to form Nanodisc around the membrane proteins. The target protein embedded Nanodiscs can then be purified.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- Supplied in nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0)
- Storage
- -20 °C,-80 °C
- Storage Comment
- Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.
- Expiry Date
- 12 months
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- Target
- G Protein-Coupled Receptor 133 (GPR133)
- Alternative Name
- ADGRD1 (GPR133 Products)
- Synonyms
- GPR133 Protein, zgc:103546 Protein, PGR25 Protein, E230012M21Rik Protein, adhesion G protein-coupled receptor D1 Protein, ADGRD1 Protein, adgrd1 Protein, Adgrd1 Protein
- Background
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GPR133, PGR25
Description: The adhesion G-protein-coupled receptors (GPCRs), including GPR133, are membrane-bound proteins with long N termini containing multiple domains. GPCRs, or GPRs, contain 7 transmembrane domains and transduce extracellular signals through heterotrimeric G proteins (summary by Bjarnadottir et al., 2004 [PubMed 15203201]).[supplied by OMIM, Nov 2010] - Molecular Weight
- The human full length ADGRD1 protein has a MW of 96.3 kDa
- UniProt
- Q6QNK2
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