BDNF Protein (Biotin)

Catalog No. ABIN7491790

Recombinant BDNF protein expressed in Escherichia coli (E. coli).

Quick Overview for BDNF Protein (Biotin) (ABIN7491790)

Target: BDNF (Brain-Derived Neurotrophic Factor (BDNF))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Application: Western Blotting (WB), Live Cell Imaging (LCI), Immunofluorescence (IF), Fluorescence Microscopy (FM), Cell Culture (CC), In vivo Studies (in vivo), Differentiation (Diff), Cytotoxicity Test (CyTox), Apoptosis Detection (AD)

Product Details

Purification tag / Conjugate: This BDNF protein is labelled with Biotin.

Purpose: human BDNF-Biotin

Sequence: HSDPARRGEL SVCDSISEWV TAADKKTAVD MSGGTVTVLE KVPVSKGQLK QYFYETKCNP MGYTKEGCRG IDKRHWNSQC RTTQSYVRAL TMDSKKRIGW RFIRIDTSCV CTLTIKRGR-OH

Specificity: Toxin target: p75 neurotrophin receptor (p75NTR), TrkB receptors

Characteristics: LC-Biotin. The extent of labeling is 1-2 Molecules of biotin per one molecule of human BDNF, linked via primary amines.

Biological Activity Comment: BDNF is a neurotrophic factor and binds p75NTR as well as TrkB receptors1,2.

Application Details

Application Notes: Antigen Preadsorption Control: ED50 = 220 pM

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the vial at 10,000 x g for 5 minutes before opening to ensure all material is collected at the bottom. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial and dissolve by gently tapping, tilting, or rolling the vial. Avoid vigorous vortexing, a brief, light vortex of 1-2 seconds is acceptable if necessary. The product is readily soluble in high-quality pure water at micromolar to millimolar concentrations (typically 5 μM to 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x the final working concentration. Divide the stock solution into small, single-use aliquots and store at -20 °C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh working solutions just before use. Avoid multiple freeze-thaw cycles to maintain optimal biological activity.

Buffer: Lyophilized from double distilled water (ddH2O). May contain residual trifluoroacetate (TFA).

Handling Advice: Centrifuge the vial at 10,000 x g for 5 minutes before opening to ensure all material is collected at the bottom,Avoid multiple freeze-thaw cycles to maintain optimal biological activity,Avoid vigorous vortexing. protect from moisture

Storage: -20 °C

Storage Comment: The product is shipped as a lyophilized powder at room temperature. Upon receipt, store at -20°C and protect from moisture.

Target Details

Target: BDNF (Brain-Derived Neurotrophic Factor (BDNF))

Alternative Name: BDNF

Background: A biotinylated human BDNF for sensitive detection of TrkB, p75NTR receptors, and the study of neuronal survival and synaptic plasticity

Molecular Weight: ~28 kDa (dimer)

Gene ID: 4915

Pathways: RTK Signaling, Synaptic Membrane, Feeding Behaviour, Dicarboxylic Acid Transport, Regulation of long-term Neuronal Synaptic Plasticity