ITCH Protein (AA 485-862)
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- Target See all ITCH Proteins
- ITCH (Itchy E3 Ubiquitin Protein Ligase Homolog (ITCH))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 485-862
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purpose
- Human ITCH Protein
- Sequence
- Arg485-Glu862
- Characteristics
- Recombinant Human ITCH Protein is expressed from E.coli without tag.It contains Arg485-Glu862.
- Purity
- > 95 % as determined by Tris-Bis PAGE,> 90 % as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Top Product
- Discover our top product ITCH Protein
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Itchy E3 Ubiquitin Protein Ligase Homolog (ITCH) (AA 197-542) protein (His-IF2DI Tag)
ITCH Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Itchy E3 Ubiquitin Protein Ligase Homolog (ITCH) (AA 92-190) protein (GST tag)ITCH Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Itchy E3 Ubiquitin Protein Ligase Homolog (ITCH) (AA 2-903) protein (His tag)Crystallography grade ITCH Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, ELISA, Crys, SDS
Itchy E3 Ubiquitin Protein Ligase Homolog (ITCH) (AA 2-864) protein (His tag)Crystallography grade ITCH Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, ELISA, Crys, SDS
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22 μm filtered solution in PBS, 200 mM L-Arginine ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- ITCH (Itchy E3 Ubiquitin Protein Ligase Homolog (ITCH))
- Alternative Name
- ITCH (ITCH Products)
- Synonyms
- AIF4 Protein, AIP4 Protein, NAPP1 Protein, dJ468O1.1 Protein, 6720481N21Rik Protein, 8030492O04Rik Protein, A130065M08 Protein, C230047C07Rik Protein, itchy E3 ubiquitin protein ligase Protein, itchy, E3 ubiquitin protein ligase Protein, ITCH Protein, Itch Protein
- Background
- Itch or itchy E3 is a member of the homologous to E6-associated protein C-terminus (HECT)-type family of E3 ligases, with the protein-interacting WW-domains for the recruitment of substrate and the HECT domain for the transfer of ubiquitin to the substrate, and its deletion results in an itchy phenotype with constant skin scratching and multi-organ inflammation.
- Molecular Weight
- 44.98 kDa same as Tris-Bis PAGE result.
- NCBI Accession
- NP_113671
- Pathways
- Activation of Innate immune Response, CXCR4-mediated Signaling Events
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