TNF alpha Protein (AA 77-233)

Catalog No. ABIN7504544

Recombinant TNF alpha protein expressed in Escherichia coli (E. coli).

Quick Overview for TNF alpha Protein (AA 77-233) (ABIN7504544)

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Protein Type: Recombinant

Biological Activity: Active

Origin: Ferret

Source: Escherichia coli (E. coli)

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 77-233

Purpose: Ferret TNF alpha Protein (active trimer)

Sequence: Val77-Leu233

Characteristics: Recombinant Ferret TNF alpha Protein is expressed from E.coli without tag.It contains Val77-Leu233.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Ferret TNF-alpha, No Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human TNFR2, hFc Tag with the EC50 of 16.6ng/ml determined by ELISA (QC Test).,Ferret TNF alpha on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Ferret TNF alpha is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22 μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Alternative Name: TNF alpha

Background: Tumor necrosis factor alpha (TNF-alpha), also known as cachectin and TNFSF2, is the prototypic ligand of the TNF superfamily. It is a pleiotropic molecule that plays a central role in inflammation, immune system development, apoptosis, and lipid metabolism.Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.

Molecular Weight: 17.21 kDa same as Bis-Tris PAGE result.

UniProt: A3FBF1

Pathways: NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome