MCEE Protein (AA 37-176) (His tag)

Catalog No. ABIN7505580

Product Details

Target: MCEE (Methylmalonyl CoA Epimerase (MCEE))

Protein Type: Recombinant

Protein Characteristics: AA 37-176

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This MCEE protein is labelled with His tag.

Sequence: Gln37-Ala176

Characteristics: Recombinant Human Methylmalonyl-CoA epimerase is produced by our Mammalian expression system and the target gene encoding Gln37-Ala176 is expressed with a 6His tag at the C-terminus.

Purity: >95 % as determined by reducing SDS-PAGE.

Endotoxin Level: <1.0 EU per μg of the protein as determined by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from sterile PBS, pH 7.4., 5 % trehalose, 5 % mannitol, 0.01 % tween-80.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: MCEE (Methylmalonyl CoA Epimerase (MCEE))

Alternative Name: MCEE (MCEE Products)

Synonyms: MGC89112 Protein, zgc:112343 Protein, MGC116480 Protein, GLOD2 Protein, 1110007A04Rik Protein, methylmalonyl-CoA epimerase Protein, methylmalonyl CoA epimerase Protein, methylmalonyl-CoA epimerase S homeolog Protein, MCEE Protein, mcee Protein, mcee.S Protein, Mcee Protein

Background:

Background: Methylmalonyl-CoA epimerase, mitochondrial(MCEE)is an enzyme which belongs to the glyoxalase I family. It converts (S)-methylmalonyl-CoA to the (R) form, catalyses the following chemical reaction: (R)-methylmalonyl-CoA (S)-methylmalonyl-CoA. It plays an important role in the catabolism of fatty acids with odd-length carbon chains. This protein deficiency is an autosomal recessive inborn error of AA metabolism, involving valine, threonine, isoleucine and methionine. This organic aciduria can appear in the neonatal period with life-threatening metabolic acidosis, hyperammonemia, feeding difficulties, pancytopenia and coma.

Synonym: Methylmalonyl-CoA epimerase, mitochondrial,DL-methylmalonyl-CoA racemase

Molecular Weight: 16.0 kDa

UniProt: Q96PE7

Pathways: Monocarboxylic Acid Catabolic Process