Myeloperoxidase Protein (MPO) (AA 49-745) (His tag)

Catalog No. ABIN7505581

Product Details

Target: Myeloperoxidase (MPO)

Protein Type: Recombinant

Protein Characteristics: AA 49-745

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This Myeloperoxidase protein is labelled with His tag.

Sequence: Ala49-Ser745

Characteristics: Recombinant Human Myeloperoxidase is produced by our Mammalian expression system and the target gene encoding Ala49-Ser745 is expressed with a 10His tag at the C-terminus.

Purity: >95 % as determined by reducing SDS-PAGE.

Endotoxin Level: <1.0 EU per μg of the protein as determined by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM Tris-HCl, 150 mM NaCl, pH 8.0.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: Myeloperoxidase (MPO)

Alternative Name: Myeloperoxidase (MPO Products)

Synonyms: mKIAA4033 Protein, MPO Protein, LOC100335032 Protein, POX2' Protein, XPOX2' Protein, mpo Protein, mpo-A Protein, pmr-1 Protein, pmr1 Protein, pox2 Protein, xpox2 Protein, myeloperoxidase Protein, myeloperoxidase L homeolog Protein, MPO Protein, Mpo Protein, LOC100335032 Protein, mpo.L Protein

Background:

Background: Myeloperoxidase (MPO) is a heme-containing enzyme belonging to the XPO subfamily of peroxidases. It is an abundant neutrophil and monocyte glycoprotein that catalyzes the hydrogen peroxide-dependent conversion of chloride, bromide, and iodide to multiple reactive species. Post-translational processing of MPO involves the insertion of a heme moiety and the proteolytic removal of both a propeptide and a 6 aa internal peptide. This results in a disulfide-linked dimer composed of a 60 kDa heavy and 12 kDa light chain that associate into a 150 kDa enzymatically active tetramer. The tetramer contains two heme groups and one disulfide bond between the heavy chains. Alternate splicing generates two additional isoforms of MPO, one with a 32 aa insertion in the light chain, and another with a deletion of the signal sequence and part of the propeptide. Human and mouse MPO share 87 % aa sequence identity. MPO activity results in protein nitrosylation and the formation of 3-chlorotyrosine and dityrosine crosslinks. MPO is also associated with a variety of other diseases, and inhibits vasodilation in inflammation by depleting the levels of NO. Serum albumin functions as a carrier protein during MPO movement to the basolateral side of epithelial cells. MPO is stored in neutrophil azurophilic granules. Upon cellular activation, it is deposited into pathogen-containing phagosomes.

Synonym: Myeloperoxidase, MPO

Molecular Weight: 80.3 kDa

UniProt: P05164

Pathways: Chromatin Binding