alpha 2 Antiplasmin Protein (His tag)

Catalog No. ABIN7534072

Product Details

Target: alpha 2 Antiplasmin (SERPINF2) (Alpha2 Antiplasmin (SERPINF2))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This alpha 2 Antiplasmin protein is labelled with His tag.

Purpose: Active Recombinant Human Serpin F2/Alpha-2-antiplasmin Protein

Sequence: MEPLGRQLTS GPNQEQVSPL TLLKLGNQEP GGQTALKSPP GVCSRDPTPE QTHRLARAMM AFTADLFSLV AQTSTCPNLI LSPLSVALAL SHLALGAQNH TLQRLQQVLH AGSGPCLPHL LSRLCQDLGP GAFRLAARMY LQKGFPIKED FLEQSEQLFG AKPVSLTGKQ EDDLANINQW VKEATEGKIQ EFLSGLPEDT VLLLLNAIHF QGFWRNKFDP SLTQRDSFHL DEQFTVPVEM MQARTYPLRW FLLEQPEIQV AHFPFKNNMS FVVLVPTHFE WNVSQVLANL SWDTLHPPLV WERPTKVRLP KLYLKHQMDL VATLSQLGLQ ELFQAPDLRG ISEQSLVVSG VQHQSTLELS EVGVEAAAAT SIAMSRMSLS SFSVNRPFLF FIFEDTTGLP LFVGSVRNPN PSAPRELKEQ QDSPGNKDFL QSLKGFPRGD KLFGPDLKLV PPMEEDYPQF GSPK

Specificity: Met28-Lys491

Purity: > 90 % by SDS-PAGE.

Sterility: 0.22 μm filtered

Endotoxin Level: < 0.1 EU/μg of the protein by LAL method.

Biological Activity Comment: Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2. The IC50 value is <0.23 nM.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1 % BSA, 5 % HSA, 10 % FBS or 5 % Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

Buffer: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

Storage: -20 °C,-80 °C

Storage Comment: Store the lyophilized protein at -20°C to -80 °C for long term.
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.

Target Details

Target: alpha 2 Antiplasmin (SERPINF2) (Alpha2 Antiplasmin (SERPINF2))

Alternative Name: Serpin F2/Alpha-2-antiplasmin (SERPINF2 Products)

Synonyms: A2AP Protein, AAP Protein, ALPHA-2-PI Protein, API Protein, PLI Protein, serpin Protein, AI747498 Protein, Pli Protein, Serpimf2 Protein, serpinf2 Protein, serpinf2l Protein, wu:fd57d08 Protein, Dmrs91 Protein, Pedf Protein, serpin family F member 2 Protein, serpin family F member 1 Protein, serine (or cysteine) peptidase inhibitor, clade F, member 2 Protein, serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 2a Protein, SERPINF2 Protein, Serpinf2 Protein, SERPINF1 Protein, serpinf2a Protein, Serpinf1 Protein

Background:

Description: SerpinF2, also known as alpha-2 antiplasmin (alpha-2 AP), is a member of the Serpin superfamily and the primary physiological inhibitor of the serine protease plasmin, which is responsible for the dissolution of fibrin clots. In addition to plasmin, Serpin F2 is also an efficient inhibitor of trypsin and chymotrypsin. This protease is produced mainly by liver and kidney, and also expressed in muscle, intestine, central nervous system, and placenta also express this protein at a moderate level.SerpinF2 is one of the inhibitors of fibrinolysis. It is a specific plasmin inhibitor, which degrades fibrin and various other proteins. Consequently, the protein plays the dominant role in regulating the blood clotting pathway. Mutations in SerpinF2 gene lead to alpha-2-plasmin inhibitor deficiency, which is characterized by severe hemorrhagic diathesis. Thus, it may be a useful target for developing more effective treatment of thrombotic diseases.

Name: SERPINF2,A2AP,AAP,ALPHA-2-PI,API,PLI

Gene ID: 5345

UniProt: P08697

Pathways: Regulation of Systemic Arterial Blood Pressure by Hormones