FCGR3B Protein (His tag)

Catalog No. ABIN7534478

Product Details

Target: FCGR3B (Fc Fragment of IgG, Low Affinity IIIb, Receptor (CD16b) (FCGR3B))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This FCGR3B protein is labelled with His tag.

Purpose: Recombinant Human Fc-gamma RIII beta/CD16b Protein

Sequence: TEDLPKAVVF LEPQWYSVLE KDSVTLKCQG AYSPEDNSTQ WFHNENLISS QASSYFIDAA TVNDSGEYRC QTNLSTLSDP VQLEVHIGWL LLQAPRWVFK EEDPIHLRCH SWKNTALHKV TYLQNGKDRK YFHHNSDFHI PKATLKDSGS YFCRGLVGSK NVSSETVNIT ITQGLAVSTI SSFSPPGYQ

Specificity: Thr20-Gln208

Purity: > 97 % by SDS-PAGE.

Sterility: 0.22 μm filtered

Endotoxin Level: < 0.1 EU/μg of the protein by LAL method.

Biological Activity Comment: Measured by its binding ability in a functional ELISA.Immobilized APC anti-human CD16 Antibody at 1μg/mL (25 μL/well) can bind Human FCGR3B with a linear range of 0.46-1.16ng/mL.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1 % BSA, 5 % HSA, 10 % FBS or 5 % Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

Buffer: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

Storage: -20 °C,-80 °C

Storage Comment: Store the lyophilized protein at -20°C to -80 °C for long term.
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.

Target Details

Target: FCGR3B (Fc Fragment of IgG, Low Affinity IIIb, Receptor (CD16b) (FCGR3B))

Alternative Name: Fc-gamma RIII beta/CD16b (FCGR3B Products)

Synonyms: CD16 Protein, CD16b Protein, FCG3 Protein, FCGR3 Protein, FCR-10 Protein, FCRIII Protein, FCRIIIb Protein, FcRIII Protein, Fc fragment of IgG receptor IIIb Protein, Fc receptor, IgG, low affinity III Protein, Fc fragment of IgG receptor IIIa Protein, Fc-gamma RIII Protein, FCGR3B Protein, Fcgr3 Protein, FCGR3A Protein, FCGR3 Protein

Background:

Description: CD16 is a low affinity Fc receptor, and has been identified as Fc receptors FcγRIIIa (CD16a) and FcγRIIIb (CD16b). These receptors function in the activation or inhibition of immune responses. CD16 encoded by two different highly homologous genes in a cell type-specific manner.CD16 is found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. CD16B is also kown as FCGR3B and FCG3B, is expressed specifically by polymorphonuclear leukocytes (neutrophils) and stimulated eosinophils. CD16B is the low affinity receptor for the Fc region of immunoglobulins gamma. CD16B may serve as a trap for immune complexes in the peripheral circulation which does not activate neutrophils.

Name: FCGR3B,CD16,CD16b,FCG3,FCGR3,FCR-10,FCRIII,FCRIIIb

Gene ID: 2215

UniProt: O75015