ICAM-3/CD50 Protein (Fc Tag,His tag)
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- Target See all ICAM-3/CD50 (ICAM3) Proteins
- ICAM-3/CD50 (ICAM3) (Intercellular Adhesion Molecule 3 (ICAM3))
- Protein Type
- Recombinant
- Biological Activity
- Active
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This ICAM-3/CD50 protein is labelled with Fc Tag,His tag.
- Purpose
- Active Recombinant Human ICAM-3/CD50 Protein
- Sequence
- QEFLLRVEPQ NPVLSAGGSL FVNCSTDCPS SEKIALETSL SKELVASGMG WAAFNLSNVT GNSRILCSVY CNGSQITGSS NITVYRLPER VELAPLPPWQ PVGQNFTLRC QVEDGSPRTS LTVVLLRWEE ELSRQPAVEE PAEVTATVLA SRDDHGAPFS CRTELDMQPQ GLGLFVNTSA PRQLRTFVLP VTPPRLVAPR FLEVETSWPV DCTLDGLFPA SEAQVYLALG DQMLNATVMN HGDTLTATAT ATARADQEGA REIVCNVTLG GERREARENL TVFSFLGPIV NLSEPTAHEG STVTVSCMAG ARVQVTLDGV PAAAPGQPAQ LQLNATESDD GRSFFCSATL EVDGEFLHRN SSVQLRVLYG PKIDRATCPQ HLKWKDKTRH VLQCQARGNP YPELRCLKEG SSREVPVGIP FFVNVTHNGT YQCQASSSRG KYTLVVVMDI EAGSSH
- Specificity
- Gln30-His485
- Purity
- > 97 % by SDS-PAGE.
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- < 0.1 EU/μg of the protein by LAL method.
- Biological Activity Comment
- Measured by the ability of the immobilized protein to support the adhesion of THP-1 cells. When cells are added to ICAM-3 coated plates (12.5 μg/mL, 100 μL/well),approximately >50% cells will adhere specifically after 60 minutes at 37 °C.
- Top Product
- Discover our top product ICAM3 Protein
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Intercellular Adhesion Molecule 3 (ICAM3) (AA 30-485) protein (His tag)
ICAM3 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
Intercellular Adhesion Molecule 3 (ICAM3) (AA 30-547) protein (GST tag)ICAM3 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Intercellular Adhesion Molecule 3 (ICAM3) (AA 30-485) protein (His tag)Crystallography grade ICAM3 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Intercellular Adhesion Molecule 3 (ICAM3) (AA 30-547) protein (rho-1D4 tag)Crystallography grade ICAM3 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1 % BSA, 5 % HSA, 10 % FBS or 5 % Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
- Buffer
- Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
- Storage
- -20 °C,-80 °C
- Storage Comment
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Store the lyophilized protein at -20°C to -80 °C for long term.
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.
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- Target
- ICAM-3/CD50 (ICAM3) (Intercellular Adhesion Molecule 3 (ICAM3))
- Alternative Name
- ICAM-3/CD50 (ICAM3 Products)
- Synonyms
- CD50 Protein, CDW50 Protein, ICAM-R Protein, ICAM-3 Protein, ICAM3 Protein, intercellular adhesion molecule 3 Protein, ICAM3 Protein, Icam3 Protein
- Background
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Description: This protein is a member of the intercellular adhesion molecule (ICAM) family. All ICAM proteins are type I transmembrane glycoproteins, contain 2-9 immunoglobulin-like C2-type domains, and bind to the leukocyte adhesion LFA-1 protein. This protein is constitutively and abundantly expressed by all leucocytes and may be the most important ligand for LFA-1 in the initiation of the immune response. It functions not only as an adhesion molecule, but also as a potent signalling molecule.
Name: ICAM3,CD50,CDW50,ICAM-R,ICAM3 / CD50
- Gene ID
- 3385
- UniProt
- P32942
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