Lipocalin 1 Protein (LCN1) (His tag)

Catalog No. ABIN7520291

Product Details

Target: Lipocalin 1 (LCN1)

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This Lipocalin 1 protein is labelled with His tag.

Purpose: Active Recombinant Human Lipocalin-1/LCN1 Protein

Sequence: HHLLASDEEI QDVSGTWYLK AMTVDREFPE MNLESVTPMT LTTLEGGNLE AKVTMLISGR CQEVKAVLEK TDEPGKYTAD GGKHVAYIIR SHVKDHYIFY CEGELHGKPV RGVKLVGRDP KNNLEALEDF EKAAGARGLS TESILIPRQS ETCSPGSD

Specificity: His19-Asp176

Purity: > 95 % by SDS-PAGE.

Sterility: 0.22 μm filtered

Endotoxin Level: <0.1EU/μg

Biological Activity Comment: Measured by its ability to inhibit active Cathepsin V cleavage of a fluorogenic peptide substrate Z-LR-AMC. The IC50 value is <2.94 nM.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1 % BSA, 5 % HSA, 10 % FBS or 5 % Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

Buffer: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

Storage: -20 °C,-80 °C

Storage Comment: Store the lyophilized protein at -20°C to -80°C for 12 months.|After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.

Target Details

Target: Lipocalin 1 (LCN1)

Alternative Name: Lipocalin-1/LCN1 (LCN1 Products)

Synonyms: PMFA Protein, TLC Protein, TP Protein, VEGP Protein, VEG Protein, lipocalin 1 Protein, odorant binding protein 2B Protein, lipocalin-1 Protein, LCN1 Protein, OBP2B Protein, Lcn1 Protein, LOC103346758 Protein

Background:

Description: Lipocalin-1, also known as Von Ebner gland protein, VEG protein, Tear Prealbumin, VEGP, Tear lipocalin, and LCN1 is a secreted protein that belongs to the calycin superfamily and Lipocalin family. Human Lipocalin-1 / VEGP was originally described as a major protein of human tear fluid, which was thought to be tear specific. Lipocalin-1 / VEGP is identical to lingual von Ebner's gland protein and is also produced in the prostate, nasal mucosa, and tracheal mucosa. Homologous proteins have been found in the rat, pig, and probably dog and horse. Lipocalin-1 / VEGP is an unusual lipocalin member, because of its high promiscuity for relative insoluble lipids and binding characteristics that differ from other members. Lipocalin-1 / VEGP acts as the principal lipid-binding protein in tear fluid, a more general physiological function has to be proposed due to its wide distribution and properties. Lipocalin-1 / VEGP would be ideally suited for scavenging of lipophilic, potentially harmful substances and thus might act as a general protection factor of epithelia. Lipocalin-1 / LCN1 could play a role in taste reception. It could be necessary for the concentration and delivery of sapid molecules in the gustatory system. Lipocalin-1 / LCN1 can bind various ligands, with chemical structures ranging from lipids and retinoids to the macrocyclic antibiotic rifampicin and even to microbial siderophores. It exhibits an extremely wide ligand pocket.

Name: LCN1,PMFA,TLC,TP,VEGP

Gene ID: 3933

UniProt: P31025