NEDD8 Protein (His tag)
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- Target See all NEDD8 Proteins
- NEDD8 (Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This NEDD8 protein is labelled with His tag.
- Purpose
- Recombinant Human NEDD8 Protein
- Sequence
- MLIKVKTLTG KEIEIDIEPT DKVERIKERV EEKEGIPPQQ QRLIYSGKQM NDEKTAADYK ILGGSVLHLV LALRGG
- Specificity
- Met1-Gly76
- Purity
- > 97 % by SDS-PAGE.
- Sterility
- 0.22 μm filtered
- Top Product
- Discover our top product NEDD8 Protein
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Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8) protein
NEDD8 Origin: Human Host: Escherichia coli (E. coli) Recombinant >95 % as determined by reducing SDS-PAGE.
Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8) (AA 1-81) protein (GST tag)NEDD8 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8) (AA 1-76) protein (His tag)Crystallography grade NEDD8 Origin: Mouse Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS, Crys
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1 % BSA, 5 % HSA, 10 % FBS or 5 % Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
- Buffer
- Lyophilized from a 0.22 μm filtered solution of 20 mM Tris, 150 mM NaCl, pH 8.0.
- Storage
- -20 °C,-80 °C
- Storage Comment
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Store the lyophilized protein at -20°C to -80 °C for long term.
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.
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- Target
- NEDD8 (Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8))
- Alternative Name
- NEDD8 (NEDD8 Products)
- Synonyms
- NEDD-8 Protein, Rub1 Protein, CG10679 Protein, Dmel\\CG10679 Protein, NEDD8 Protein, dNEDD8 Protein, nedd8 Protein, ubiquitin Protein, si:zc14a17.5 Protein, GB17785 Protein, DDBDRAFT_0218116 Protein, DDBDRAFT_0238041 Protein, DDB_0218116 Protein, DDB_0238041 Protein, nedd8.S Protein, neural precursor cell expressed, developmentally down-regulated 8 Protein, neural precursor cell expressed, developmentally down-regulated gene 8 Protein, CG10679 gene product from transcript CG10679-RB Protein, Nedd8 Protein, ubiquitin-like protein Nedd8 Protein, neural precursor cell expressed, developmentally down-regulated 8 L homeolog Protein, NEDD8 Protein, Nedd8 Protein, nedd8 Protein, nedd8.L Protein
- Background
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Description: Neural Precursor Cell Expressed Developmentally Downregulated Gene 8 (NEDD8), also known as Related to Ubiquitin 1 (Rub1), is a 6-8 kDa member of the Ubiquitin family of proteins. Human NEDD8 is activated by a distinct NEDD8-activating (E1) enzyme, a heterodimeric complex composed of APPBP1 and UBA3 subunits . Activated NEDD8 is subsequently transferred to the UBE2M/Ubc12 or UBE2F NEDD8-conjugating (E2) enzymes . Through a process termed neddylation, the ROC1/Rbx1 RING Finger E3 ligase transfers NEDD8 to specific substrates. NEDD8 plays a critical regulatory role in cell proliferation and dysregulation of the NEDD8 pathway has been associated with several cancer pathologies .
Name: NEDD8,NEDD-8
- Gene ID
- 4738
- UniProt
- Q15843
- Pathways
- Ubiquitin Proteasome Pathway
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