Olfactomedin 4 Protein (OLFM4) (His tag)

Catalog No. ABIN7520372

Product Details

Target: Olfactomedin 4 (OLFM4)

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This Olfactomedin 4 protein is labelled with His tag.

Purpose: Recombinant human Olfactomedin-4/OLFM4 Protein

Sequence: DLGDVGPPIP SPGFSSFPGV DSSSSFSSSS RSGSSSSRSL GSGGSVSQLF SNFTGSVDDR GTCQCSVSLP DTTFPVDRVE RLEFTAHVLS QKFEKELSKV REYVQLISVY EKKLLNLTVR IDIMEKDTIS YTELDFELIK VEVKEMEKLV IQLKESFGGS SEIVDQLEVE IRNMTLLVEK LETLDKNNVL AIRREIVALK TKLKECEASK DQNTPVVHPP PTPGSCGHGG VVNISKPSVV QLNWRGFSYL YGAWGRDYSP QHPNKGLYWV APLNTDGRLL EYYRLYNTLD DLLLYINARE LRITYGQGSG TAVYNNNMYV NMYNTGNIAR VNLTTNTIAV TQTLPNAAYN NRFSYANVAW QDIDFAVDEN GLWVIYSTEA STGNMVISKL NDTTLQVLNT WYTKQYKPSA SNAFMVCGVL YATRTMNTRT EEIFYYYDTN TGKEGKLDIV MHKMQEKVQS INYNPFDQKL YVYNDGYLLN YDLSVLQKPQ

Specificity: Asp21-Gln510

Sterility: 0.22 μm filtered

Endotoxin Level: <1EU/μg

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1 % BSA, 5 % HSA, 10 % FBS or 5 % Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

Concentration: 0.43 mg/mL

Buffer: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

Storage: -20 °C,-80 °C

Storage Comment: Store the lyophilized protein at -20°C to -80°C for 12 months.|After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.

Target Details

Target: Olfactomedin 4 (OLFM4)

Alternative Name: Olfactomedin-4/OLFM4 (OLFM4 Products)

Synonyms: tiarin Protein, GC1 Protein, GW112 Protein, OLM4 Protein, OlfD Protein, UNQ362 Protein, bA209J19.1 Protein, hGC-1 Protein, hOLfD Protein, Gm296 Protein, Gm913 Protein, pPD4 Protein, olfactomedin-4 Protein, olfactomedin 4 L homeolog Protein, olfactomedin 4 Protein, olfactomedin-4-like Protein, olfm4.L Protein, OLFM4 Protein, olfm4 Protein, LOC100225360 Protein, Olfm4 Protein

Background:

Description: Olfactomedin-4 (OLFM4), previously called GC-1 (G-CSF stimulated clone-1) is an secreted glycoprotein that is a member of the Olfactomedin/Noelin family of protein . It is expressed in epithelial cells of the prostate, small intestine, colon, bone marrow, and in several cancers , and is up-regulated in epithelial cells during inflammation . Mature OLFM4 is a 490 amino acid (aa) protein that is secreted mainly in a polymeric form which is held together by disulfide bonds and carbohydrate interactions . Mature human OLFM4 shares 71 % aa sequence identity with mature mouse and rat OLFM4. Olfactomedin-4 (OLFM4) interacts with cell surface lectins and cadherins, which helps cell adhesion and cell spreading of NIH3T3 and 293T/17 cells . OLFM4 plays a critical role in regulating progression of prostate cancer and is also novel biomarker for triple-negative breast cancer .

Name: GC1, OLM4, OlfD, GW112, hGC-1, hOLfD, UNQ362, bA209J19.1

Gene ID: 10562

UniProt: Q6UX06