Podoplanin Protein (PDPN) (Fc Tag,His tag)

Catalog No. ABIN7534258

Product Details

Target: Podoplanin (PDPN)

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This Podoplanin protein is labelled with Fc Tag,His tag.

Purpose: Active Recombinant Human Podoplanin/PDPN Protein

Sequence: EGASTGQPED DTETTGLEGG VAMPGAEDDV VTPGTSEDRY KSGLTTLVAT SVNSVTGIRI EDLPTSESTV HAQEQSPSAT ASNVATSHST EKVDGDTQTT VEK

Specificity: Glu97-Lys199

Purity: > 95 % by SDS-PAGE.

Sterility: 0.22 μm filtered

Endotoxin Level: < 0.1 EU/μg of the protein by LAL method.

Biological Activity Comment: Measured by its binding ability in a functional ELISA. Immobilized Human CLEC-2 Protein at 5 μg/mL (100 μL/well) can bind Human Podoplanin with a linear range of 0.98-46 ng/mL.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1 % BSA, 5 % HSA, 10 % FBS or 5 % Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

Buffer: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

Storage: -20 °C,-80 °C

Storage Comment: Store the lyophilized protein at -20°C to -80 °C for long term.
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.

Target Details

Target: Podoplanin (PDPN)

Alternative Name: Podoplanin/PDPN (PDPN Products)

Synonyms: PDPN Protein, AGGRUS Protein, GP36 Protein, GP40 Protein, Gp38 Protein, HT1A-1 Protein, OTS8 Protein, PA2.26 Protein, T1A Protein, T1A-2 Protein, OTS-8 Protein, RANDAM-2 Protein, T1-alpha Protein, T1a Protein, T1alpha Protein, E11 Protein, RTI40 Protein, podoplanin Protein, PDPN Protein, Pdpn Protein

Background:

Description: Podoplanin, also known as glycoprotein 36 (gp36), PA2.26 antigen, T1-alpha (T1A), and aggrus, is a 36 kDa type I transmembrane sialoglycoprotein and member of the Podoplanin family.PDPN is a mucin-type glycoprotein negatively charged by extensive O-glycosylation and a high content of sialic acid, which expresses the adhesive property. It is selectively expressed in lymphatic endothelium as well as lymphangiomas, Kaposi sarcomas, and in a subset of angiosarcomas with probable lymphatic differentiation. PDPN may contribute to form odontoblastic fiber or function as the anchorage to the tooth development and in proliferating epithelial cells of cervical loop and apical bud. The intensity of podoplanin expression is negatively correlated with the expression of CD34 and factor VIII. Podoplanin would be useful as a diagnostic marker for epithelioid hemangioendothelioma in liver tumors.

Name: AGGRUS, GP36, GP40, Gp38, HT1A-1, OTS8, PA2.26, T1A, T1A-2, T1A2, TI1A,PDPN, AGGRUS, podoplanin,GP36,GP40,Gp38,HT1A-1,OTS8,PA2.26,T1A,T1A-2,T1A2,TI1A

Gene ID: 10630

UniProt: Q86YL7-3

Pathways: Dicarboxylic Acid Transport