TWEAK Protein
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- Target See all TWEAK (TNFSF12) Proteins
- TWEAK (TNFSF12) (Tumor Necrosis Factor (Ligand) Superfamily, Member 12 (TNFSF12))
- Protein Type
- Recombinant
- Biological Activity
- Active
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purpose
- Active Recombinant Human TNFSF12/TWEAK Protein
- Sequence
- KGRKTRARRA IAAHYEVHPR PGQDGAQAGV DGTVSGWEEA RINSSSPLRY NRQIGEFIVT RAGLYYLYCQ VHFDEGKAVY LKLDLLVDGV LALRCLEEFS ATAASSLGPQ LRLCQVSGLL ALRPGSSLRI RTLPWAHLKA APFLTYFGLF QVH
- Specificity
- Lys97-His249(N-Met)
- Purity
- > 95 % by SDS-PAGE.
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- <0.1EU/μg
- Biological Activity Comment
- 1.Measured by its binding ability in a functional ELISA. Immobilized Human TNFSF12 at 2 μg/mL (100 μL/well) can bind Human TNFRSF12A with a linear range of 0.1-2.3 ng/mL.|2.Measured in a cell proliferation assay using human umbilical vein endothelial cells (HUVEC). The ED50 for this effect is typically 0.81-3.24 ng/mL.
- Top Product
- Discover our top product TNFSF12 Protein
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Tumor Necrosis Factor (Ligand) Superfamily, Member 12 (TNFSF12) (AA 43-249) protein (Fc Tag)
TNFSF12 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Tumor Necrosis Factor (Ligand) Superfamily, Member 12 (TNFSF12) (AA 106-249) protein (His-DYKDDDDK-Strep II Tag)TNFSF12 Origin: Human Host: HEK-293T Cells Recombinant SDS, WB, ELISA, PI
Tumor Necrosis Factor (Ligand) Superfamily, Member 12 (TNFSF12) (AA 49-134) protein (GST tag)TNFSF12 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Tumor Necrosis Factor (Ligand) Superfamily, Member 12 (TNFSF12) (AA 1-134) protein (GST tag)TNFSF12 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Tumor Necrosis Factor (Ligand) Superfamily, Member 12 (TNFSF12) (AA 43-249) protein (His tag)Crystallography grade TNFSF12 Origin: Human Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Tumor Necrosis Factor (Ligand) Superfamily, Member 12 (TNFSF12) (AA 46-249) protein (His tag)Crystallography grade TNFSF12 Origin: Mouse Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Tumor Necrosis Factor (Ligand) Superfamily, Member 12 (TNFSF12) (AA 1-249) protein (Strep Tag)Crystallography grade TNFSF12 Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA
Tumor Necrosis Factor (Ligand) Superfamily, Member 12 (TNFSF12) (AA 1-249) protein (Strep Tag)Crystallography grade TNFSF12 Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA
Tumor Necrosis Factor (Ligand) Superfamily, Member 12 (TNFSF12) (AA 94-249) protein (rho-1D4 tag)Crystallography grade TNFSF12 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Tumor Necrosis Factor (Ligand) Superfamily, Member 12 (TNFSF12) (AA 94-249) protein (rho-1D4 tag)Crystallography grade TNFSF12 Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1 % BSA, 5 % HSA, 10 % FBS or 5 % Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
- Buffer
- Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
- Storage
- -20 °C,-80 °C
- Storage Comment
- Store the lyophilized protein at -20°C to -80°C for long term.|After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.
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- Target
- TWEAK (TNFSF12) (Tumor Necrosis Factor (Ligand) Superfamily, Member 12 (TNFSF12))
- Alternative Name
- TNFSF12/TWEAK (TNFSF12 Products)
- Synonyms
- APO3L Protein, DR3LG Protein, TWEAK Protein, Apo3l Protein, Dr3l Protein, Dr3lg Protein, Tweak Protein, TNF superfamily member 12 Protein, tumor necrosis factor (ligand) superfamily, member 12 Protein, TNFSF12 Protein, Tnfsf12 Protein
- Background
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Description: TNFSF12 is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. It is a ligand for the FN14/TWEAKR receptor. TNFSF12 has overlapping signaling functions with TNF, but displays a much wider tissue distribution. It can induce apoptosis via multiple pathways of cell death in a cell type-specific manner. It is also found that TNFSF12 promotes proliferation and migration of endothelial cells, and thus acts as a regulator of angiogenesis. TNFSF12 also is a weak inducer of apoptosis in some cell types and mediates NF-kappa-B activation.
Name: TNFSF12,APO3L,DR3LG,TNLG4A,TWEAK
- Gene ID
- 8742
- UniProt
- O43508-1
- Pathways
- Apoptosis
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