Leptin Protein (LEP) (AA 22-167) (Fc Tag)

Catalog No. ABIN7538352

This Recombinant Leptin protein is expressed in Mammalian Cells.

Quick Overview for Leptin Protein (LEP) (AA 22-167) (Fc Tag) (ABIN7538352)

Target: Leptin (LEP)

Protein Type: Recombinant

Origin: Human

Source: Mammalian Cells

Purity: The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.

Product Details

Protein Characteristics: AA 22-167

Purification tag / Conjugate: This Leptin protein is labelled with Fc Tag.

Purpose: Recombinant human LEP Protein with C-terminal human Fc tag

Specificity: LEP (Val22-Cys167) hFc (Glu99-Ala330)

Characteristics: Extracellular Domain Protein

Purification: Purified from cell culture supernatant by affinity chromatography

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose is added as protectants before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Expiry Date: 12 months

Target Details

Target: Leptin (LEP)

Alternative Name: LEP

Background: This gene encodes a protein that is secreted by white adipocytes into the circulation and plays a major role in the regulation of energy homeostasis. Circulating leptin binds to the leptin receptor in the brain, which activates downstream signaling pathways that inhibit feeding and promote energy expenditure. This protein also has several endocrine functions, and is involved in the regulation of immune and inflammatory responses, hematopoiesis, angiogenesis, reproduction, bone formation and wound healing. Mutations in this gene and its regulatory regions cause severe obesity and morbid obesity with hypogonadism in human patients. A mutation in this gene has also been linked to type 2 diabetes mellitus development. [provided by RefSeq, Aug 2017]

Molecular Weight: predicted molecular mass of 42.2 kDa after removal of the signal peptide. The apparent molecular mass of LEP-hFc is 35-55 kDa due to glycosylation.

UniProt: P41159

Pathways: JAK-STAT Signaling, AMPK Signaling, Hormone Transport, Peptide Hormone Metabolism, Hormone Activity, Negative Regulation of Hormone Secretion, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Monocarboxylic Acid Catabolic Process