TNF alpha Protein

Catalog No. ABIN7539350

This Recombinant TNF alpha protein is expressed in Escherichia coli (E. coli).

Quick Overview for TNF alpha Protein (ABIN7539350)

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 95 % by SDS-PAGE and RP-HPLC analysis

Product Details

Purpose: TNF-alpha

Sequence: MVRSSSRTPS DKPVAHVVAN PQAEGQLQWL NRRANALLAN GVELRDNQLV VPSEGLYLIY SQVLFKGQGC PSTHVLLTHT ISRIAVSYQT KVNLLSAIKS PCQRETPEGA E AKPWYEPIY LGGVFQLEKG DRLSAEINRP DYLDFAESGQ VYFGIIAL

Specificity: Chromosomal location:6p21.3

Characteristics: Length (aa):158

Application Details

Application Notes: The ED50 as determined by the proliferation assay with TF-1 cells is in the range of 0.05-0.4 ng/mL.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: The lyophilized TNF-alpha is soluble in water and most aqueous buffers. The lyophilized powder can be reconstituted in sterile water to a concentration of 0.1 mg/mL. This solution can be diluted into other buffered solutions or stored at -20 °C for future use.

Buffer: 20 mM PB, ph 7.2 + 100 mM NaCl

Storage: RT,4 °C,-20 °C

Storage Comment: Lyophilized Tumor Necrosis Factor-alpha although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TNF-alpha should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).

Target Details

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Alternative Name: TNF-alpha

Background: TNF, DIF, TNFA, TNFSF2, TNF-alpha,Tumor necrosis factor is a cytokine involved in systemic inflammation and is a member of a group of cytokines that all stimulate the acute phase reaction. TNF is mainly secreted by macrophages. TNF causes apoptotic cell death, cellular proliferation, differentiation, inflammation, tumorigenesis and viral replication, TNF is also involved in lipid metabolism, and coagulation. TNF's primary role is in the regulation of immune cells. Dysregulation and, in particular, overproduction of TNF have been implicated in a variety of human diseases- autoimmune diseases, insulin resistance, and cancer. Human recombinant Tumor Necrosis Factor-α produced in E. coli is a single, non-glycosylated, polypeptide chain containing 158 amino acids and having a molecular mass of 17483.77 Dalton.

Molecular Weight: 17.5 kDa

Gene ID: 7124

NCBI Accession: NM_000594, NP_000585

UniProt: P01375

Pathways: NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome