HSP27 Protein (AA 1-209) (His tag)
-
- Target See all HSP27 (HSPB1) Proteins
- HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-209
-
Origin
- Mouse
-
Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This HSP27 protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Sequence
-
MTERRVP FSLLRSPSWE PFRDWYPAHS RLFDQAFGVP RLPDEWSQWF SAAGWPGYVR PLPAATAEGP AAVTLAAPAF SRALNRQLSS GVSEIRQTAD RWRVSLDVNH FAPEELTVKT KEGVVEITGK HEERQDEHGY ISRCFTRKYT LPPGVDPTLV SSSLSPEGTL TVEAPLPKAV TQSAEITIPV TFEARAQIGG PEAGKSEQSG AK - Purity
- > 90% by SDS-PAGE
- Top Product
- Discover our top product HSPB1 Protein
-
Heat Shock 27kDa Protein 1 (HSPB1) (AA 1-209) protein (His tag)
custom-made HSPB1 Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Heat Shock 27kDa Protein 1 (HSPB1) (AA 1-205) protein (His tag)custom-made HSPB1 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Heat Shock 27kDa Protein 1 (HSPB1) (AA 1-205) protein (GST tag)HSPB1 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Heat Shock 27kDa Protein 1 (HSPB1) proteinHSPB1 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % pure SDS
Heat Shock 27kDa Protein 1 (HSPB1) proteinHSPB1 Origin: Mouse Host: Escherichia coli (E. coli) Recombinant > 95 % ELISA, SDS, WB, IP
Heat Shock 27kDa Protein 1 (HSPB1) protein (Myc-DYKDDDDK Tag)HSPB1 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
-
- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- 1 mg/mL
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.
-
- Target
- HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))
- Alternative Name
- HSP27 (HSPB1 Products)
- Background
- HSP27, also known as heat shock protein beta-1, is one of the small heat shock proteins and constitutively expressed in various tissues. HSP27 expression is increased to high levels after various types of stress including elevated temperatures, toxic metals, drugs and oxidants. HSP27 and its phosphorylation state participate in the regulation of multiple physiological and pathophysiological cell functions. However, the exact roles of HSP27 in osteoblasts remain unclear. In the present study, we investigated the role of HSP27 in the platelet-derived growth factor-BB(PDGF-BB)-stimulated migration of osteoblast-like MC3T3-E1 cells. Recombinant mouse HSP27, fused to His-tag at N-terminus, was expressed in E.coli and purified by conventional chromatography techniques.
- Molecular Weight
- 25.4 kDa (232aa) confirmed by MALDI-TOF
- NCBI Accession
- NP_038588
- Pathways
- MAPK Signaling, Regulation of Actin Filament Polymerization, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, VEGF Signaling
-
