HSP27 Protein (AA 1-205) (His tag)

Catalog No. ABIN7504421

Product Details

Target: HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))

Protein Type: Recombinant

Protein Characteristics: AA 1-205

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This HSP27 protein is labelled with His tag.

Purpose: Human Hsp27/HSPB1 Protein

Sequence: Met1-Lys205

Characteristics: Recombinant Human Hsp27/HSPB1 Protein is expressed from E.coli with His tag at the C-Terminus.It contains Met1-Lys205.

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS, 2 mM DTT ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Preservative: Dithiothreitol (DTT)

Precaution of Use: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))

Alternative Name: Hsp27 (HSPB1 Products)

Synonyms: CMT2F Protein, HMN2B Protein, HS.76067 Protein, HSP27 Protein, HSP28 Protein, Hsp25 Protein, SRP27 Protein, 27kDa Protein, Hsp27 Protein, cb153 Protein, cb660 Protein, hsp1 Protein, hsp25 Protein, hsp27 Protein, id:ibd2821 Protein, sb:cb660 Protein, zgc:103437 Protein, HSPB1 Protein, cmt2f Protein, hs.76067 Protein, hsp28 Protein, heat shock protein family B (small) member 1 Protein, heat shock protein 1 Protein, heat shock protein, alpha-crystallin-related, 1 Protein, heat shock protein family B (small) member 1 L homeolog Protein, HSPB1 Protein, Hspb1 Protein, hspb1 Protein, hspb1.L Protein

Background: Heat shock protein beta-1 (HSPB1, also known as HSP27) is a small heat shock protein involved in many cellular processes and reportedly protects cells against oxidative stress. This protein is expressed only in insulin-dependent tissues (heart, skeletal muscle, and fat tissue), and expression of HspB7 is regulated by many different factors. HspB7 has an unusual N-terminal sequence, a conservative α-crystallin domain, and very short C-terminal domain lacking conservative IPV tripeptide involved in a small heat shock proteins oligomer formation.

Molecular Weight: 23.74 kDa. The protein migrates to 25-30 kDa based on Tris-Bis PAGE result.

NCBI Accession: NP_001531

Pathways: MAPK Signaling, Regulation of Actin Filament Polymerization, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, VEGF Signaling