EPH Receptor A3 Protein (EPHA3) (AA 21-324) (His tag)

Catalog No. ABIN7597350

Product Details

Target: EPH Receptor A3 (EPHA3)

Protein Type: Recombinant

Protein Characteristics: AA 21-324

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This EPH Receptor A3 protein is labelled with His tag.

Purpose: Recombinant human EPHA3(21-324) Protein with C-terminal 10xHis tag

Sequence: EPHA3(Glu21-Arg324) 10xHis tag

Purity: The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.

Application Details

Application Notes: Extracellular Domain Proteins (ECD) can be used as:

• Immunogens for antibody drug development
• Reagents used for CAR-T positive cell monitoring
• Reagents for antibody screening and functional testing
• Reagents for antibody affinity measurement

Comment:

The protein was made using HEK293 mammalian cell secretion expression system to ensure the close-to-native structures and post-translational modifications of the target protein.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from sterile PBS, pH 7.4. Normally 5 % – 8% trehalose is added as protectants before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Expiry Date: 12 months

Target Details

Target: EPH Receptor A3 (EPHA3)

Alternative Name: EPHA3 (EPHA3 Products)

Background: EK4, ETK, HEK, ETK1, HEK4, TYRO4
This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. This gene encodes a protein that binds ephrin-A ligands. Two alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Jul 2008]

Molecular Weight: predicted molecular mass of 36.0 kDa after removal of the signal peptide. The apparent molecular mass of EPHA3(21-324)-His is 35-55 kDa due to glycosylation.

UniProt: P29320

Pathways: RTK Signaling, Regulation of Cell Size