Ephrin B3 Protein (EFNB3) (AA 28-224) (His tag)

Catalog No. ABIN7678623

This Recombinant Ephrin B3 protein is produced in Mammalian Cells.

Quick Overview for Ephrin B3 Protein (EFNB3) (AA 28-224) (His tag) (ABIN7678623)

Target: Ephrin B3 (EFNB3)

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Mammalian Cells

Application: SDS-PAGE (SDS), ELISA, Western Blotting (WB), Activity Assay (AcA), Immunogen (Imm)

Purity: >90 % as determined by SDS-PAGE.

Product Details

Protein Characteristics: AA 28-224

Purification tag / Conjugate: This Ephrin B3 protein is labelled with His tag.

Purpose: Recombinant Human EFNB3 Protein, C-His (Active)

Sequence: Leu28-Ser224

Endotoxin Level: <0.1 EU/μg of the protein by the LAL method.

Biological Activity Comment: Measured by its binding ability in a functional ELISA. When recombinant human EphB3 is coated at 2 μg/mL, recombinant human EFNB3 (ABIN7607572) binds with an apparent Kd < 0.4 nM.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.

Buffer: Lyophilized from a solution in PBS pH 7.4, 5 % Trehalose, 5 % Mannitol.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for one week. Store at -20 to -80°C for twelve months from the date of receipt.

Expiry Date: 12 months

Target Details

Target: Ephrin B3 (EFNB3)

Alternative Name: Ephrin-B3

Background: Ephrin-B3, LERK8, EPLG8, EFNB3, EPH-related receptor transmembrane ligand ELK-L3, EPH-related receptor tyrosine kinase ligand 8, LERK-8

UniProt: Q15768

Pathways: RTK Signaling