IFN omega (Active) protein (His tag)

Catalog No. ABIN7668916

This Recombinant IFN omega protein is produced in Escherichia coli (E. coli).

Quick Overview for IFN omega (Active) protein (His tag) (ABIN7668916)

Target: IFN omega

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Application: Cell Culture (CC)

Purity: > 95 %

Product Details

Purification tag / Conjugate: His tag

Purpose: Recombinant Human IFN omega protein(His Tag)

Sequence: Cys 24-Ser 195

Sterility: 0.2 μm filtered

Endotoxin Level: < 0.1 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Measure by its ability to induce cytotoxicity in TF-1 cells. The ED₅₀ for this effect is <0.02 ng/mL. The specific activity of recombinant human IFN omega is approximately >5 x10⁷ IU/ mg.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: IFN omega

Background: Interferon omega-1 is also known as Interferon alpha-II-1and IFNW1. It is a Secreted protein that in humans is encoded by the IFNW1 gene. IFNW1 belongs to the alpha/beta interferon family. Type I IFNs consist of IFN α, β, τ, and ω and bind to the type I IFN receptor, whereas IFN-γ is the only type II IFN and is specific for the type II IFN receptor. IFNW1 is a recently discovered protein structurally related to IFN-alpha and -beta. It has been shown that IFN-omega 1 similar to that of other human class I IFNs, potent antiviral activity was also observed on cells of bovine and ovine but not of equine or murine origin.,IFN alpha II-1,IFNW1

Molecular Weight:

calculated_mw: 20.9 kDa

observed_mw: 20 kDa

UniProt: P05000