IL-10 Protein (His tag)

Catalog No. ABIN7669005

Recombinant IL-10 protein expressed in HEK-293 Cells.

Quick Overview for IL-10 Protein (His tag) (ABIN7669005)

Target: IL-10 (IL10) (Interleukin 10 (IL10))

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Application: Immunogen (Imm)

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Purification tag / Conjugate: This IL-10 protein is labelled with His tag.

Purpose: Recombinant Mouse IL10 Protein (His Tag)

Sequence: Met1-178Ser

Purification: > 95 % as determined by reducing SDS-PAGE.

Sterility: 0.2 μm filtered

Endotoxin Level: < 1.0 EU/mg of the protein as determined by the LAL method

Biological Activity Comment: Not validated for activity

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with 5 % Trehalose and 5 % Mannitol.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: IL-10 (IL10) (Interleukin 10 (IL10))

Alternative Name: IL10

Background: Interleukin,IL-,CSIF,If2a,Il-10,IL10A,TGIF,Interleukin-10,IL10,Cytokine synthesis inhibitory factor,N/A,CSIF,If2a,Il-10,Mouse Il10 is the prototypic member of the IL-10 cytokine family, including IL-10, IL-19, IL-20, IL-22 (IL-TIF), IL-24 and IL-26. Many viruses encode viral members of the IL-10 family, such as Epstein-Barr virus (EBV) and human cytomegalovirus (HCMV).Its main function is inhibiting the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells. Although human and mouse IL-10 are 81 % identical at the nucleotide and amino acid level, mouse IL-10 is species-specific and does not act on human cells. Interestingly, Human IL-10 is active on mouse cells.

Molecular Weight:

Calculated MW: 19.5 kDa

Observed MW: 22 kDa

Gene ID: 16153

UniProt: P18893

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location, Cancer Immune Checkpoints