NOV Protein (Fc Tag)

Catalog No. ABIN7669350

Recombinant NOV protein expressed in Mammalian Cells.

Quick Overview for NOV Protein (Fc Tag) (ABIN7669350)

Target: NOV (Nephroblastoma Overexpressed (NOV))

Protein Type: Recombinant

Origin: Mouse

Source: Mammalian Cells

Application: Immunogen (Imm)

Purity: > 90 % as determined by reducing SDS-PAGE.

Product Details

Purification tag / Conjugate: This NOV protein is labelled with Fc Tag.

Purpose: Recombinant Mouse NOV/CCN3 Protein(Fc Tag)

Sequence: Gln22-lle354

Purification: SDS-PAGE analysis of Mouse NOV/CCN3 proteins, 2 μg/lane of Recombinant Mouse NOV/CCN3 proteins was resolved with SDS-PAGE under reducing conditions, showing bands at 80 KD

Sterility: 0.2 μm filtered

Endotoxin Level: < 1.0 EU/mg of the protein as determined by the LAL method

Biological Activity Comment: Not validated for activity

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with 5 % Trehalose and 5 % Mannitol.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: NOV (Nephroblastoma Overexpressed (NOV))

Alternative Name: NOV

Background: CCN,CCN family member,NovH,CCN family member 3,Nephroblastoma-overexpressed gene protein homolog,Protein NOV homolog,Nov,Ccn3,Protein NOV homolog, also known as Nephroblastoma-overexpressed gene protein homolog, NOV, and CCN3, is a putative ligand for integrin receptors, is tightly associated with the extracellular matrix, and mediates diverse cellular functions, including cell adhesion and proliferation. CCN3 has been shown to negatively regulate growth althoμgh it promotes migration in a cell type-specific manner. This secreted protein belongs to the CCN family, and its expression was observed in a broad variety of tissues from the early stage of development, and altered expression of CCN3 has been observed in a variety of tumors, including hepatocellular carcinomas, Wilm's tumors, Ewing's sarcomas, gliomas, rhabdomyosarcomas, and adrenocortical carcinomas. Mature CCN3 protein has five distinct modules and truncated protein variants with altered function are found in many cancers. CCN3 acts throμgh the core stem cell signaling pathways including Notch and Bone Morphogenic Protein, connecting CCN3 with the modulation of self-renewal and maturation of some cell lineages including hematopoietic, osteogenic, and chondrogenic. CCN3 may affect the extracellular environment of the niche for hematopoietic stem cells. CCN3 has emerged as a key player in stem cell regulation, hematopoiesis, and a crucial component within the bone marrow microenvironment.

Molecular Weight:

Calculated MW: 61.5 kDa

Observed MW: 80 kDa

UniProt: Q64299

Pathways: Smooth Muscle Cell Migration, Growth Factor Binding