SARS-CoV-2 Spike Protein (AA 319-541, Receptor Binding Domain)

Catalog No. ABIN7669692

Recombinant SARS-CoV-2 Spike protein expressed in HEK-293 Cells.

Quick Overview for SARS-CoV-2 Spike Protein (AA 319-541, Receptor Binding Domain) (ABIN7669692)

Target: SARS-CoV-2 Spike

Protein Type: Recombinant

Biological Activity: Active

Origin: SARS Coronavirus-2 (SARS-CoV-2)

Source: HEK-293 Cells

Application: ELISA

Purity: > 95 %

Product Details

Protein Characteristics: AA 319-541, Receptor Binding Domain

Purpose: Recombinant SARS-CoV-2 Spike Protein (RBD)

Sequence: Arg319-Phe541

Purification: > 95 % as determined by reducing SDS-PAGE.

Sterility: 0.2 μm filtered

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Immobilized Recombinant 2019-nCoV Spike Protein (RBD)(PKSR030521) at 2 μg/mL (100 μL/well) can bind ACE2 Protein, Recombinant Human ACE2 Protein (mFc Tag) (PKSR030509), the EC50 of ACE2 Protein, Recombinant Human ACE2 Protein (mFc Tag) (PKSR030509) is 20-80 ng/mL.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: SARS-CoV-2 Spike

Target Type: Viral Protein

Background: The spike (S) glycoprotein of coronaviruses contains protrusions that will only bind to certain receptors on the host cell. Known receptors bind S1 are ACE2, angiotensin-converting enzyme 2, DPP4, dipeptidyl peptidase-4, APN, aminopeptidase N, CEACAM, carcinoembryonic antigen-related cell adhesion molecule 1, Sia, sialic acid, O-ac Sia, O-acetylated sialic acid. The spike is essential for both host specificity and viral infectivity. The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. It's been reported that SARS-CoV-2 (COVID-19 coronavirus, 2019-nCoV) can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion. The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. The main functions for the Spike protein are summarized as: Mediate receptor binding and membrane fusion, Defines the range of the hosts and specificity of the virus, Main component to bind with the neutralizing antibody, Key target for vaccine design, Can be transmitted between different hosts through gene recombination or mutation of the receptor binding domain (RBD), leading to a higher mortality rate.,NCP-CoV RBD,NCP-CoV Spike,NCP-CoV s1,NCP-CoV s2,RBD,S1,S2,Spike RBD,coronavirus spike,cov spike,ncov RBD,ncov s1,ncov s2,ncov spike,novel coronavirus RBD,novel coronavirus s1,novel coronavirus s2,novel coronavirus spike

Molecular Weight: 25.1 kDa