HMGB1 Protein (BoxAB, C-Tailless)

Catalog No. ABIN7862612

Recombinant HMGB1 protein expressed in Escherichia coli (E. coli).

Quick Overview for HMGB1 Protein (BoxAB, C-Tailless) (ABIN7862612)

Target: HMGB1 (High Mobility Group Box 1 (HMGB1))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: Activation (Act), Cellular Assay (CA)

Purity: > 95 % It contains no nucleic acids.

Product Details

Protein Characteristics: BoxAB, C-Tailless

Purpose: BoxAB (Tailless-HMGB1) is a recombinant fragment of HMGB1 including both DNA-binding domains, BoxA and BoxB, lacking the acidic C-terminal tail.

Sequence: BoxAB from HMGB1 consists of 176 amino acids: MGKGDPKKPR GKMSSYAFFV QTCREEHKKK HPDASVNFSE FSKKCSERWK TMSAKEKGKF EDMAKADKAR YEREMKTYIP PKGETKKKFK DPNAPKRPPS AFFLFCSEYR PKIKGEHPGL SIGDVAKKLG EMWNNTAADD KQPYEKKAAK LKEKYEKDIA AYRAKGKPDA AKKGVV

Characteristics: BoxAB from HMGB1 includes both BoxA and BoxB domains connected as in the natural sequence but lacks the negatively charged C-terminal tail. This tailless form retains the structural integrity and receptor-binding capacity of full-length HMGB1, while showing altered functional behavior in redox-dependent signaling and immune activation.
BoxAB is ideal for studying the mechanisms of HMGB1-mediated inflammation, receptor binding (RAGE, TLR2, TLR4), and DNA interaction without interference from the acidic tail. The protein is fully compatible with in vitro biochemical assays, cell culture studies, and receptor-ligand binding analyses.
The product is produced in E.coli from an expression plasmid coding for the human HMGB1 sequence (amino acids 1–176) without any tags or modifications, ensuring natural folding and charge distribution.

Purification: Purified. Oxidation of cysteine 106 makes the protein inactive. To avoid cysteine oxidation DTT 0.5 mM is added during protein purification.

Endotoxin Level: LPS-free: Pierce™ Chromogenic Endotoxin Quant Kit, <0.1 EU/mL

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: The lyophilized protein should be reconstituted in distilled water, resulting in a solution containing 50 mM HEPES pH 7.9, 500 mM NaCl, 0.5 mM DTT.

Buffer: The protein is lyophilized from 50 mM HEPES pH 7.9, 500 mM NaCl, 0.5 mM DTT.
The product contains contains < 0.006% v/v of Triton X-114 due to LPS removal. Remaining traces can be removed upon request.

Storage: 4 °C/-20 °C

Storage Comment: 2-8°C when lyophilized. The protein once reconstituted with water can be stored frozen (-20°C).

Target Details

Target: HMGB1 (High Mobility Group Box 1 (HMGB1))

Alternative Name: High-mobility group protein B1 (HMGB1)

Background: HMGB1 is a nuclear protein that is released passively by necrotic cells, retained by apoptotic cells, and secreted actively by inflammatory cells.

Molecular Weight: 20.0 kDa

Pathways: p53 Signaling, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Toll-Like Receptors Cascades, Smooth Muscle Cell Migration, Inflammasome