IL-15 Protein

Catalog No. ABIN7865994

This Recombinant IL-15 protein is produced in Escherichia coli (E. coli).

Quick Overview for IL-15 Protein (ABIN7865994)

Target: IL-15 (IL15) (Interleukin 15 (IL15))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: Escherichia coli (E. coli)

Purity: >95 % , as determined by SDS-PAGE and HPLC

Product Details

Purpose: Mouse IL15 Protein Recombinant

Sequence: MKILKPYMRN TSISCYLCFL LNSHFLTEAG IHVFILGCVS VGLPKTEANW IDVRYDLEKI ESLIQSIHID TTLYTDSDFH PSCKVTAMNC FLLELQVILH EYSNMTLNET VRNVLYLANS TLSSNKNVAE SGCKECEELE EKTFTEFLQS FIRIVQMFIN TS

Characteristics:

Protein Size: Native mouse Interleukin-15 is generated by the proteolytic removal of the signal peptide and propeptide, the molecule has a calculated molecular mass of approximately 13 kDa. Recombinant mouse Interleukin-15 is a disulfide-linked homodimeric protein consisting of 115 amino acid residue subunits, and migrates as an approximately 13 kDa protein under non-reducing conditions and reducing conditions in SDS-PAGE.

Sequence Position: Optimized DNA sequence encoding mouse Interleukin-15 mature chain was expressed in Escherichia Coli.

Sterility: 0.2 μm filtered

Endotoxin Level: Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/μg(1EU/μg).

Biological Activity Comment: The ED(50) was determined by the dose-dependent proliferation of murine CTLL-2 cells is < 2.0 ng/ml.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

Buffer: Recombinant mouse IL-15 was lyophilized from a 0.2 μm filtered PBS solution.

Storage: 4 °C,-20 °C

Storage Comment: The lyophilized protein is stable for at least 2 years from date of receipt at -20°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2°- 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

Expiry Date: 24 months

Target Details

Target: IL-15 (IL15) (Interleukin 15 (IL15))

Alternative Name: IL15

Molecular Weight: 13kDa

Pathways: JAK-STAT Signaling, Glycosaminoglycan Metabolic Process