Midkine Protein (AA 21-143)
Quick Overview for Midkine Protein (AA 21-143) (ABIN7866037)
Target
See all Midkine (MDK) ProteinsProtein Type
Biological Activity
Origin
Source
Purity
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Protein Characteristics
- AA 21-143
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Purpose
- Human MDK Protein Recombinant
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Sequence
- VAKKKDKVKK GGPGSECAEW AWGPCTPSSK DCGVGFREGT CGAQTQRIRC RVPCNWKKEF GADCKYKFEN WGACDGGTGT KVRQGTLKKA RYNAQCQETI RVTKPCTPKT KAKAKAKKGK GKD
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Characteristics
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Protein Size: Recombinant MDK migrates as an approximately 14 kDa protein under reducing conditions in SDS-PAGE.
Sequence Position: Optimized DNA sequence encoding Human MDK mature chain was expressed in E. Coli. Recombinant Human Midkine encodes amino acid residues 21-143.
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Endotoxin Level
- Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/μg(1EU/μg).
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Biological Activity Comment
- Determined by its ability to chemoattract human neutrophils using a concentration range of 0.1-10.0 ng/ml
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Midkine (Neurite Growth-Promoting Factor 2) (MDK) (Active) protein
MDK Origin: Human Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS-PAGE Func Active
Midkine (Neurite Growth-Promoting Factor 2) (MDK) (AA 1-143) protein (GST tag)MDK Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Midkine (Neurite Growth-Promoting Factor 2) (MDK) (AA 23-143) protein (Fc Tag)MDK Origin: Cynomolgus Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. AM, ADA, IA, ScA
Midkine (Neurite Growth-Promoting Factor 2) (MDK) (AA 21-143) protein (Fc Tag)MDK Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. AM, ADA, IA, ScA
Midkine (Neurite Growth-Promoting Factor 2) (MDK) (AA 23-140) (Active) protein (His tag)MDK Origin: Mouse Host: Escherichia coli (E. coli) Recombinant > 95 % as determined by Bis-Tris PAGE Active
Midkine (Neurite Growth-Promoting Factor 2) (MDK) (AA 21-143) (Active) protein (His-Avi Tag)MDK Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % as determined by Bis-Tris PAGE Active
Midkine (Neurite Growth-Promoting Factor 2) (MDK) (AA 21-140) protein (His-IF2DI Tag)MDK Origin: Mouse Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Midkine (Neurite Growth-Promoting Factor 2) (MDK) (AA 21-143) protein (His tag)MDK Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % SDS
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
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Buffer
- MDK was Lyophilized from PBS, pH 7.2.
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Storage
- 4 °C,-20 °C
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Storage Comment
- The lyophilized protein is stable for at least 2 years from date of receipt at -20°C. Upon reconstitution, this product can be stored in working aliquots at 2°- 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
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Expiry Date
- 24 months
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- Midkine (MDK) (Midkine (Neurite Growth-Promoting Factor 2) (MDK))
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Alternative Name
- MDK
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Molecular Weight
- 14 kDa
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Pathways
- RTK Signaling, M Phase, Skeletal Muscle Fiber Development
Target
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