Oncostatin M Protein (OSM) (AA 26-208)
Quick Overview for Oncostatin M Protein (OSM) (AA 26-208) (ABIN7866045)
Target
See all Oncostatin M (OSM) ProteinsProtein Type
Biological Activity
Origin
Source
Purity
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Protein Characteristics
- AA 26-208
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Purpose
- Rat OSM Protein Recombinant
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Sequence
- MKRGCSSSSP KLLSQLKSQA NITGNTASLL EPYILHQNLN TLTLRAACTE HPVAFPSEDM LRQLSKPDFL STVHATLGRV WHQLGAFRQQ FPKIQDFPEL ERARQNIQGI RNNVYCMARL LHPPLEIPEP TQADSGTSRP TTTAPGIFQI KIDSCRFLWG YHRFMGSVGR VFEEWGDGSR RSRRHSPLWA WLKGDHRIRP SRSSQSAMLR SLVPR
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Characteristics
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Protein Size: Recombinant OSM migrates as an approximately 24 kDa protein under reducing conditions in SDS-PAGE.
Sequence Position: Optimized DNA sequence encoding Rat OSM mature chain was expressed in E. Coli. Recombinant Rat Oncostatin-M encodes amino acid residues 26-208.
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Endotoxin Level
- Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/μg(1EU/μg).
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Biological Activity Comment
- Determined by its ability to stimulate the proliferation of rat C6 cells. The expected ED50 is 3.0-5.0 μg/ml.
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Oncostatin M (OSM) (AA 26-221) protein (His-Avi Tag)
OSM Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Oncostatin M (OSM) (AA 26-221) (Active) protein (Biotin,His-Avi Tag)OSM Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
Oncostatin M (OSM) (AA 25-206) (Active) protein (His tag)OSM Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
Oncostatin M (OSM) (AA 1-252) protein (GST tag)OSM Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Oncostatin M (OSM) protein (His tag)OSM Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by reducing SDS-PAGE. AM, ADA, IA, ScA
Oncostatin M (OSM) (AA 26-221) (Active) protein (His tag)OSM Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA Active
Oncostatin M (OSM) (AA 26-239) protein (His-IF2DI Tag)OSM Origin: Rat Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Oncostatin M (OSM) (AA 26-234) protein (His tag)OSM Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % SDS
Oncostatin M (OSM) (AA 26-252) proteinOSM Origin: Human Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
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Buffer
- OSM was Lyophilized from PBS, pH 7.2.
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Storage
- 4 °C,-20 °C
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Storage Comment
- The lyophilized protein is stable for at least 2 years from date of receipt at -20°C. Upon reconstitution, this product can be stored in working aliquots at 2°- 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
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Expiry Date
- 24 months
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- Oncostatin M (OSM)
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Alternative Name
- OSM
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Molecular Weight
- 24 kDa
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Pathways
- JAK-STAT Signaling, Negative Regulation of Hormone Secretion
Target
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