Thrombopoietin Protein (THPO) (AA 22-326)
Quick Overview for Thrombopoietin Protein (THPO) (AA 22-326) (ABIN7866069)
Target
See all Thrombopoietin (THPO) ProteinsProtein Type
Biological Activity
Origin
Source
Purity
-
-
Protein Characteristics
- AA 22-326
-
Purpose
- Rat THPO Protein Recombinant
-
Sequence
- SPVPPACDPR LLNKLLRDSY LLHSRLSQCP DVNPLSIPVL LPAVDFSLGE WKTQTEQSKA QDILGAVSLL LEGVMAARGQ LEPSCLSSLL GQLSGQVRLL LGALQGLLGT QLPPQGRTTA HKDPSALFLS LQQLLRGKVR FLLLVEGPAL CVRRTLPTTA VPSRTSQLLT LNKF
-
Characteristics
-
Protein Size: Recombinant THPO migrates as an approximately 19 kDa protein due to glycosylation under reducing conditions in SDS-PAGE.
Sequence Position: Optimized DNA sequence encoding Rat THPO mature chain was expressed in CHO. Recombinant Rat Thrombopoietin encodes amino acid residues 22-326.
-
Endotoxin Level
- Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/μg(1EU/μg).
-
Biological Activity Comment
- Determined by its ability to stimulate the proliferation of human MO7e cells. The expected ED50 is ≤ 0.2 ng/ml, corresponding to a specific activity of ≥ 5 x 106 units/mg.
-
-
Want other Options for this Protein ?
!Discover Our Predefined Custom Proteins and Custom Protein Services!Your project requires further customization? Contact us and discover our custom protein solutions
-
Thrombopoietin (THPO) protein (His tag)
THPO Origin: Mouse Host: Human Cells Recombinant > 95 % as determined by SDS-PAGE
Thrombopoietin (THPO) (AA 23-195) protein (His tag)THPO Origin: Mouse Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Thrombopoietin (THPO) (AA 22-195) protein (His-IF2DI Tag)THPO Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Thrombopoietin (THPO) (AA 1-349) protein (GST tag)THPO Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Thrombopoietin (THPO) (AA 22-356) (Active) protein (His tag)THPO Origin: Mouse Host: Mammalian Cells Recombinant >90 % as determined by SDS-PAGE. SDS, WB, Imm, ELISA, AcA Active
Thrombopoietin (THPO) (AA 22-356) (Active) proteinTHPO Origin: Mouse Host: CHO Cells Recombinant > 95 % as analyzed by SDS-PAGE. Active
Thrombopoietin (THPO) proteinTHPO Origin: Mouse Host: Escherichia coli (E. coli) Recombinant 97,00% SDS
Thrombopoietin (THPO) proteinTHPO Origin: Mouse Host: Escherichia coli (E. coli) Recombinant 97,00% SDS
-
-
Restrictions
- For Research Use only
-
-
-
Format
- Lyophilized
-
Reconstitution
- A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
-
Buffer
- THPO was Lyophilized from PBS, pH 7.2.
-
Storage
- 4 °C,-20 °C
-
Storage Comment
- The lyophilized protein is stable for at least 2 years from date of receipt at -20°C. Upon reconstitution, this product can be stored in working aliquots at 2°- 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
-
Expiry Date
- 24 months
-
-
- Thrombopoietin (THPO)
-
Alternative Name
- THPO
-
Molecular Weight
- 19 kDa
-
Pathways
- JAK-STAT Signaling, Hormone Activity
Target
-
