MGMT Protein (His tag)
Quick Overview for MGMT Protein (His tag) (ABIN7935595)
Target
See all MGMT ProteinsProtein Type
Origin
Source
Purity
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Purification tag / Conjugate
- This MGMT protein is labelled with His tag.
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Purpose
- Recombinant Human 6-O-Methylguanine-DNA Methyltransferase/MGMT (N-6His)
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Sequence
- MGSSHHHHHH SSGLVPRGSH MDKDCEMKRT TLDSPLGKLE LSGCEQGLHE IKLLGKGTSA ADAVEVPAPA AVLGGPEPLM QCTAWLNAYF HQPEAIEEFP VPALHHPVFQ QESFTRQVLW KLLKVVKFGE VISYQQLAAL AGNPKAARAV GGAMRGNPVP ILIPCHRVVC SSGAVGNYSG GLAVKEWLLA HEGHRLGKPG LGGSSGLAGA WLKGAGATSG SPPAGRN
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Sterility
- 0.2 μm filtered
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Endotoxin Level
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.
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O6-Methylguanine-DNA-Methyltransferase (MGMT) (AA 1-207) protein (His tag)
custom-made MGMT Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
O6-Methylguanine-DNA-Methyltransferase (MGMT) (AA 1-207) protein (GST tag)MGMT Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
O6-Methylguanine-DNA-Methyltransferase (MGMT) (AA 1-207) protein (His-IF2DI Tag)MGMT Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.< br ,>It is not recommended to reconstitute to a concentration less than 100 μ,g,ml.< br ,>Dissolve the lyophilized protein in ddH2O.< br ,>Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
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Buffer
- Supplied as a 0.2 um filtered solution of 20 mMTrisHCl,1 mM EDTA, pH 8.0 .
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Handling Advice
- Please minimize freeze-thaw cycles
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Storage
- -20 °C
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Storage Comment
- Store at < -20°C, stable for 6 months after receipt.Please minimize freeze-thaw cycles.
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Expiry Date
- 6 months
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- MGMT (O6-Methylguanine-DNA-Methyltransferase (MGMT))
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Alternative Name
- 6-O-Methylguanine-DNA Methyltransferase/MGMT
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Molecular Weight
- 23.8kDa
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Gene ID
- 4255
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UniProt
- P16455
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Pathways
- DNA Damage Repair, Positive Regulation of Response to DNA Damage Stimulus
Target
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