Sonic Hedgehog Protein (SHH)

Catalog No. ABIN7936273

Recombinant Sonic Hedgehog protein expressed in Escherichia coli (E. coli).

Quick Overview for Sonic Hedgehog Protein (SHH) (ABIN7936273)

Target: Sonic Hedgehog (SHH)

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purity: Greater than 95 % as determined by reducing SDS-PAGE.

Product Details

Purpose: Recombinant Human Sonic Hedgehog/SHH C24II

Sequence: MIIGPGRGFG KRRHPKKLTP LAYKQFIPNV AEKTLGASGR YEGKISRNSE RFKELTPNYN PDIIFKDEEN TGADRLMTQR CKDKLNALAI SVMNQWPGVK LRVTEGWDED GHHSEESLHY EGRAVDITTS DRDRSKYGML ARLAVEAGFD WVYYESKAHI HCSVKAENSV AAKSGG

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.< br ,>It is not recommended to reconstitute to a concentration less than 100 μ,g,ml.< br ,>Dissolve the lyophilized protein in ddH2O.< br ,>Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Lyophilized from a 0.2 um filtered solution of 20 mM PB, 100 mM NaCl, 1 mM DTT, pH 7.5.

Preservative: Dithiothreitol (DTT)

Precaution of Use: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7°C for 2-7 days.Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 3 weeks

Target Details

Target: Sonic Hedgehog (SHH)

Alternative Name: Sonic Hedgehog/SHH C24II

Molecular Weight: 19.8kDa

Gene ID: 6469

UniProt: Q15465

Pathways: Hedgehog Signaling, Dopaminergic Neurogenesis, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development