TNF alpha Protein
Quick Overview for TNF alpha Protein (ABIN7937246)
Target
See all TNF alpha ProteinsProtein Type
Biological Activity
Origin
Source
Purity
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Purpose
- Recombinant Rat Tumor Necrosis Factor-alpha/TNFSF2
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Sequence
- LRSSSQNSSD KPVAHVVANH QAEEQLEWLS QRANALLANG MDLKDNQLVV PADGLYLIYS QVLFKGQGCP DYVLLTHTVS RFAISYQEKV SLLSAIKSPC PKDTPEGAEL KPWYEPMYLG GVFQLEKGDL LSAEVNLPKY LDITESGQVY FGVIAL
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Sterility
- 0.2 μm filtered
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Endotoxin Level
- Less than 1 EU/ug of rRtTNF-alpha as determined by LAL method.
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Biological Activity Comment
- Fully biologically active when compared to standard. The ED50 as determined by a cytotoxicity assay using murine L929 cells is less than 0.05 ng/ml, corresponding to a specific activity of > 2.0 x107 IU/mg in the presence of actinomycin D.
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Tumor Necrosis Factor alpha (TNF alpha) (AA 77-233) (Active) protein (Biotin,His-Avi Tag)
TNF alpha Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
Tumor Necrosis Factor alpha (TNF alpha) (AA 78-233) protein (His tag)TNF alpha Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. AM, ADA, IA, ScA
Tumor Necrosis Factor alpha (TNF alpha) (AA 77-233) (Active) protein (His-Avi Tag)TNF alpha Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
Tumor Necrosis Factor alpha (TNF alpha) (AA 77-233) (Active) protein (Biotin)TNF alpha Origin: Human Host: HEK-293 Cells Recombinant >95 % as determined by reduced SDS-PAGE. Active
Tumor Necrosis Factor alpha (TNF alpha) (AA 77-233) (Active) protein (His tag,AVI tag,Biotin)TNF alpha Origin: Human Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
Tumor Necrosis Factor alpha (TNF alpha) (AA 77-233) (Active) proteinHPLC verified TNF alpha Origin: Human Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
Tumor Necrosis Factor alpha (TNF alpha) (AA 1-233) protein (GST tag)TNF alpha Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Tumor Necrosis Factor alpha (TNF alpha) (Active) proteinTNF alpha Origin: Rabbit Host: Escherichia coli (E. coli) Recombinant > 95 % as determined by reducing SDS-PAGE. Active
Tumor Necrosis Factor alpha (TNF alpha) (Active) proteinTNF alpha Origin: Mouse Host: Escherichia coli (E. coli) Recombinant > 95 % as determined by SDS-PAGE Active
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Comment
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Approximately 17.2 kDa, a single non-glycosylated polypeptide chain containing 156 amino acids.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Buffer
- Lyophilized from a 0.2 um filtered concentrated solution in 20 mM PB, pH 7.2, 150 mM NaCl.
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Storage
- 4 °C,-20 °C,-80 °C
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Storage Comment
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Expiry Date
- 12 months
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- TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))
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Alternative Name
- Tumor Necrosis Factor-alpha
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Molecular Weight
- 17.2 kDa
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Gene ID
- 24835
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UniProt
- P16599
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Pathways
- NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome
Target
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