Sonic Hedgehog Protein (SHH)

Catalog No. ABIN7937272

Recombinant Sonic Hedgehog protein expressed in Escherichia coli (E. coli).

Quick Overview for Sonic Hedgehog Protein (SHH) (ABIN7937272)

Target: Sonic Hedgehog (SHH)

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Purity: >98 % as determined by SDS-PAGE and HPLC.

Product Details

Purpose: Recombinant Human Sonic hedgehog protein(SHH) (Active)

Sequence: IVIGPGRGFG KRRHPKKLTP LAYKQFIPNV AEKTLGASGR YEGKISRNSE RFKELTPNYN PDIIFKDEEN TGADRLMTQR CKDKLNALAI SVMNQWPGVK LRVTEGWDED GHHSEESLHY EGRAVDITTS DRDRSKYGML ARLAVEAGFD WVYYESKAHI HCSVKAENSV AAKSGG

Sterility: 0.2 μm filtered

Biological Activity Comment: Fully biologically active when compared to standard. The ED50 as determined by inducing alkaline phosphatase production of murine C3H,10T1,2 cells is less than 1 g,ml, corresponding to a specific activity of 1.0 103 IU,mg.

Application Details

Comment:

19.8 kDa

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 0.2 um filtered 20 mM PB, pH 7.4, 150 mM NaCl ,lyophilized

Handling Advice: Repeated freezing and thawing is not recommended

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C,-80°C. The shelf life of lyophilized form is 12 months at -20°C,-80°C.
Notes:Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Expiry Date: 12 months

Target Details

Target: Sonic Hedgehog (SHH)

Alternative Name: Sonic hedgehog

Molecular Weight: 19.8 kDa

Gene ID: 6469

UniProt: Q15465

Pathways: Hedgehog Signaling, Dopaminergic Neurogenesis, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development