Sonic Hedgehog Protein (SHH) (N-Term)

Catalog No. ABIN7937273

This Recombinant Sonic Hedgehog protein is produced in Escherichia coli (E. coli).

Quick Overview for Sonic Hedgehog Protein (SHH) (N-Term) (ABIN7937273)

Target: Sonic Hedgehog (SHH)

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 98 % by SDS-PAGE and HPLC analyses.

Product Details

Protein Characteristics: N-Term

Purpose: Recombinant Human Sonic Hedgehog N-Terminus

Sequence: IVIGPGRGFG KRRHPKKLTP LAYKQFIPNV AEKTLGASGR YEGKISRNSE RFKELTPNYN PDIIFKDEEN TGADRLMTQR CKDKLNALAI SVMNQWPGVK LRVTEGWDED GHHSEESLHY EGRAVDITTS DRDRSKYGML ARLAVEAGFD WVYYESKAHI HCSVKAENSV AAKSGG

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 1 EU/ug of rHuSHH as determined by LAL method.

Biological Activity Comment: Fully biologically active when compared to standard. The ED50 as determined by inducing alkaline phosphatase production of murine C3H/10T1/2 cells is less than 1 μg/ml, corresponding to a specific activity of > 1.0 x10³ IU/mg.

Application Details

Comment:

Approximately 19.8 kDa, a single non-glycosylated polypeptide chain containing 176 amino acids.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from a 0.2 um filtered concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Expiry Date: 12 months

Target Details

Target: Sonic Hedgehog (SHH)

Alternative Name: Sonic Hedgehog

Molecular Weight: 19.8 kDa

Gene ID: 6469

UniProt: Q15465

Pathways: Hedgehog Signaling, Dopaminergic Neurogenesis, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development