NOG Protein
Quick Overview for NOG Protein (ABIN7937428)
Target
See all NOG ProteinsProtein Type
Biological Activity
Origin
Source
Purity
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Purpose
- Recombinant Human Noggin, Insect Cells Derived
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Sequence
- QHYLHIRPAP SDNLPLVDLI EHPDPIFDPK EKDLNETLLR SLLGGHYDPG FMATSPPEDR PGGGGGAAGG AEDLAELDQL LRQRPSGAMP SEIKGLEFSE GLAQGKKQRL SKKLRRKLQM WLWSQTFCPV LYAWNDLGSR FWPRYVKVGS CFSKRSCSVP EGMVCKPSKS VHLTVLRWRC QRRGGQRCGW IPIQYPIISE CKCSC
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Sterility
- 0.2 μm filtered
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Endotoxin Level
- Less than 0.1 EU/ug of rHuNoggin, Insect Cell as determined by LAL method.
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Biological Activity Comment
- Measured by its ability to inhibit BMP-4-induced alkaline phosphatase production by ATDC5 mouse chondrogenic cells. The ED50 for this effect is 0.04-0.2 μg/mL in the presence of 50 ng/mL of Recombinant Human BMP-4.
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Noggin (NOG) (AA 20-232) (Active) protein
NOG Origin: Mouse Host: CHO Cells Recombinant > 95 % as analyzed by SDS-PAGE. Active
Noggin (NOG) (AA 28-232) (Active) proteinNOG Origin: Human Host: CHO Cells Recombinant > 95 % as analyzed by SDS-PAGE. Active
Noggin (NOG) (AA 28-232) protein (GST tag)NOG Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Noggin (NOG) protein (His tag)NOG Origin: Mouse Host: Human Cells Recombinant > 95 % as determined by reducing SDS-PAGE.
Noggin (NOG) protein (Fc Tag)NOG Origin: Human Host: HEK-293 Cells Recombinant Greater than 95 % as determined by reducing SDS-PAGE. AM, ADA, IA, ScA
Noggin (NOG) (AA 28-232) protein (His-IF2DI Tag)NOG Origin: Mouse Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Noggin (NOG) (AA 28-232) protein (Fc Tag)NOG Origin: Human Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE.
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Comment
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Approximately 23.0 kDa on SDS-PAGE under reducing conditions, containing 205 amino acids, and a molecular mass about 47.9 kDa homodimer under non-reducing conditions (Molecular size on SDS-PAGE will appear at approximately 50-80 kDa).
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Buffer
- Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4, 5 % trehalose, 0.02 %Tween-20.
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Storage
- 4 °C,-20 °C,-80 °C
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Storage Comment
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Expiry Date
- 12 months
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- NOG (Noggin (NOG))
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Alternative Name
- Noggin
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Molecular Weight
- 23.0 kDa,47.9 kDa,80 kDa
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Gene ID
- 9241
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UniProt
- Q13253
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Pathways
- Stem Cell Maintenance, Tube Formation
Target
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