EPH Receptor A4 Protein (EPHA4) (AA 570-986)

Catalog No. ABIN7938798

This Recombinant EPH Receptor A4 protein is produced in Baculovirus infected Insect Cells.

Quick Overview for EPH Receptor A4 Protein (EPHA4) (AA 570-986) (ABIN7938798)

Target: EPH Receptor A4 (EPHA4)

Protein Type: Recombinant

Origin: Human

Source: Baculovirus infected Insect Cells

Application: Screening Assay (ScA), Functional Studies (Func), Activity Assay (AcA)

Product Details

Protein Characteristics: AA 570-986

Purpose: Recombinant EPHA4 (570-986) protein

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Recombinant EPHA4 (570-986) protein is supplied in 25 mM HEPES pH 7.5, 300 mM NaCl, 20 % glycerol, 0.04 % Triton X-100, 0.5 mM TCEP.

Handling Advice: Avoid repeated freeze/thaw cycles,keep on ice when not in storage

Storage: -80 °C

Storage Comment: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Target Details

Target: EPH Receptor A4 (EPHA4)

Alternative Name: EPHA4

Background:

Protein Background: EPHA4 (Ephrin type-A receptor 4) is a receptor tyrosine kinase which binds membrane-bound ephrin family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Highly promiscuous, it has the unique property among Eph receptors to bind and to be physiologically activated by both GPI-anchored ephrin-A and transmembrane ephrin-B ligands including EFNA1 and EFNB3. Upon activation by ephrin ligands, modulates cell morphology and integrin-dependent cell adhesion through regulation of the Rac, Rap and Rho GTPases activity. Plays an important role in the development of the nervous system controlling different steps of axonal guidance including the establishment of the corticospinal projections. May also control the segregation of motor and sensory axons during neuromuscular circuit development. In addition to its role in axonal guidance plays a role in synaptic plasticity. Activated by EFNA1 phosphorylates CDK5 at 'Tyr-15' which in turn phosphorylates NGEF regulating RHOA and dendritic spine morphogenesis. In the nervous system, also plays a role in repair after injury preventing axonal regeneration and in angiogenesis playing a role in central nervous system vascular formation.

Short Description: Recombinant EPHA4 (570-986) protein that includes amino acids 570-986 of human EPHA4 protein (accession number NP_001291465.1) was expressed in a baculovirus expression system and contains an N-terminal FLAG tag. The molecular weight of the protein is 48.46 kDa.

Aliases: Ephrin type-A receptor 4, EPH Receptor A4, HEK8, TYRO1, Tyrosine-Protein Kinase Receptor SEK, EK8

Pathways: RTK Signaling