EPH Receptor B2 Protein (EPHB2) (AA 570-987)

Catalog No. ABIN7938803

This Recombinant EPH Receptor B2 protein is produced in Baculovirus infected Insect Cells.

Quick Overview for EPH Receptor B2 Protein (EPHB2) (AA 570-987) (ABIN7938803)

Target: EPH Receptor B2 (EPHB2)

Protein Type: Recombinant

Origin: Human

Source: Baculovirus infected Insect Cells

Application: Activity Assay (AcA), Screening Assay (ScA), Functional Studies (Func)

Product Details

Protein Characteristics: AA 570-987

Purpose: Recombinant EPHB2 (570-987) protein

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Recombinant EPHB2 (570-987) protein is supplied in 25 mM HEPES pH 7.5, 300 mM NaCl, 20 % glycerol, 0.04 % Triton X-100, 0.5 mM TCEP.

Handling Advice: Avoid repeated freeze/thaw cycles,keep on ice when not in storage

Storage: -80 °C

Storage Comment: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Target Details

Target: EPH Receptor B2 (EPHB2)

Alternative Name: EPHB2

Background:

Protein Background: EPHB2 is a receptor tyrosine kinase,has a prototypical RTK topology including an N-terminal multidomain extracellular region, a membrane spanning region, and an intracellular region.EPHB2 binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. Functions in axon guidance during development. Involved in the guidance of commissural axons, that form a major interhemispheric connection between the 2 temporal lobes of the cerebral cortex. Also involved in guidance of contralateral inner ear efferent growth cones at the midline and of retinal ganglion cell axons to the optic disk. In addition to axon guidance, also regulates dendritic spines development and maturation and stimulates the formation of excitatory synapses. Upon activation by EFNB1, abolishes the ARHGEF15-mediated negative regulation on excitatory synapse formation. Controls other aspects of development including angiogenesis, palate development and in inner ear development through regulation of endolymph production. Forward and reverse signaling through the EFNB2/EPHB2 complex regulate movement and adhesion of cells that tubularize the urethra and septate the cloaca. May function as a tumor suppressor. May be involved in the regulation of platelet activation and blood coagulation.

Short Description: Recombinant EPHB2 (570-987) protein that includes amino acids 570-987 of human EPHB2 protein (accession number NP_004433.2) was expressed in a baculovirus expression system, and contains an N-terminal FLAG tag. The molecular weight of the protein is 48.65 kDa.

Aliases: EPH receptor B2, EPHT3, HEK5, DRT, ERK, Hek5, Tyro5

Pathways: RTK Signaling, Regulation of long-term Neuronal Synaptic Plasticity, S100 Proteins