HNMT Protein
Quick Overview for HNMT Protein (ABIN7938959)
Target
See all HNMT ProteinsProtein Type
Origin
Source
Application
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Purpose
- Recombinant HNMT protein
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Histamine N-Methyltransferase (HNMT) (AA 1-292) protein (His tag)
custom-made HNMT Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Histamine N-Methyltransferase (HNMT) (AA 1-295) protein (His tag)custom-made HNMT Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Histamine N-Methyltransferase (HNMT) (AA 1-51) protein (GST tag)HNMT Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Histamine N-Methyltransferase (HNMT) (AA 1-292) protein (His tag)HNMT Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % SDS
Histamine N-Methyltransferase (HNMT) (AA 1-292) (Active) protein (His tag)HNMT Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % SDS, EAA Active
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Liquid
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Buffer
- Recombinant HNMT protein is supplied in 25 mM Tris-HCl pH 8.0, 300 mM NaCl, 10 % glycerol and 0.5 mM TCEP.
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Handling Advice
- Avoid repeated freeze/thaw cycles,keep on ice when not in storage
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Storage
- -80 °C
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Storage Comment
- Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
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- HNMT (Histamine N-Methyltransferase (HNMT))
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Alternative Name
- HNMT
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Background
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Protein Background: Histamine acts as a neurotransmitter in the brain which participates in the regulation of many biological processes including inflammation, gastric acid secretion, and neuromodulation. The enzyme histamine N-methyltransferase (HNMT) inactivates histamine by transferring a methyl group from S-adenosyl-L-methionine to histamine, and is the only well-known pathway for termination of neurotransmission actions of histamine in mammalian CNS.
Short Description: Recombinant HNMTprotein that includes full lengthof human HNMT protein (accession number NP_008826.1) was expressed was expressed in E. coli cells with an N-terminal GST tag. The molecular weight of the protein is 60.13 kDa.
Aliases: Histamine N-methyltransferase, HMT, MRT51
Target
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